NCT00775424已完成1 期
A PHASE Ib PARTIALLY RANDOMIZED PILOT STUDY INTENDED TO EVALUATE THE SAFETY AND IMMUNOLOGICAL EFFECTS OF HIV-1 DNA IMMUNIZATION (PENNVAX-B) WITH OR WITHOUT CO-ADMINISTRATION OF CONSTRUCTS CONTAINING DNA ENCODING FOR THE EXPRESSION OF EITHER IL-12 OR IL-15 IN HIV INFECTED INDIVIDUALS
适应症
试验速览
- 阶段
- 1 期
- 状态
- 已完成
- 入组人数
- 38
- 试验地点
- 2
- 主要终点
- Frequency of adverse events in each of the treatment arms
研究概览
简要总结
A phase Ib partially blinded pilot study to evaluate the safety and immunological effects of PENNVAX-B with or without co-administration of constructs containing DNA encoding for the expression of either IL-12 or IL-15.
Primary objectives
- To determine the safety of HIV-1 DNA constructs (PENNVAX-B).
- To determine the safety and optimal doses of the IL-12 and the IL-15 adjuvant constructs when given with PENNVAX-B.
Secondary objectives
- To compare the various vaccine groups for their immunological responses to several HIV-1 antigens, utilizing the ELISPOT assay.
- To analyze antibody responses to the vaccine antigens over time.
- To measure CD8 cell proliferative responses to vaccine antigens over time.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Treatment
- 盲法
- Quadruple (Participant, Care Provider, Investigator, Outcomes Assessor)
入排标准
- 年龄范围
- 18 Years 至 50 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •HIV-1 infection documented by any licensed ELISA test kit and confirmed by Western blot, HIV-1 culture, HIV-1 antigen, or plasma HIV-1 RNA, at any time before study entry.
- •Taking a stable HAART regimen for ≥3 months before the time of enrollment.
- •CD4-positive lymphocyte count ≥400 cells/µl on two occasions within 60 days of enrollment, performed at any certified flow laboratory.
- •HIV-1 < 75 copies/mL on two occasions within 60 days of enrollment, performed in a CLIA certified laboratory.
- •Laboratory values obtained within 30 days prior to study entry:
- •Hemoglobin > 9 g/dL (female subjects) and > 9.5 g/dL (male subjects)
- •Absolute neutrophil count > 1000 cells/μL
- •Platelet count > 75,000/μL
- •ALT, AST and alkaline phosphatase ≥ 2.5 x upper limit of normal range
- •Total bilirubin ≥ 2.5 x upper limit of the laboratory normal range
- •Serum creatinine ≥ upper limit of normal (ULN).
- •All women of reproductive potential (who have not reached menopause nor undergone hysterectomy, oophorectomy, or tubal ligation) must have a negative pregnancy test within 30 days of initiating study-specified medication(s) and at day 0 (enrollment).
- •Women who are not of reproductive potential (have reached menopause or undergone hysterectomy, oophorectomy, or tubal ligation) or whose male partner has undergone successful vasectomy with resultant azoospermia or has azoospermia for any other reason, are eligible without requiring the use of contraception. Documentation of menopause, sterilization (hysterectomy, oophorectomy, tubal ligation, or vasectomy) and azoospermia by patient-reported history is acceptable.
- •All subjects must not participate in a conception process (i.e. active attempt to become pregnant or to impregnate, sperm donation, in vitro fertilization), and if participating in sexual activity that could lead to pregnancy, the female study volunteer/male partner must use a form of contraception while receiving protocol-specified medication(s)/vaccinations and for one month after stopping the vaccinations.
- •Karnofsky performance score ≥ 90 within 30 days prior to study entry.
- •Men or women ≥18 years of age and less than
- •Ability and willingness of subject or legal guardian/representative to give written informed consent.
排除标准
- •Any active or past AIDS-defining illness with the exception of minimal (less than 10 lesions) cutaneous Kaposi's sarcoma.
- •Subjects with a history of a CD4+ T-cell count ≤200/µl are not eligible.
- •Use of any known immunomodulatory therapy within 4 weeks prior to study entry including but not limited to drugs such as systemic corticosteroids, interferons, interleukins, thalidomide, granulocyte-macrophage colony-stimulating factor, IV gammaglobulin, or human growth hormone.
- •Any malignancy requiring systemic or local toxic chemotherapy. Local radiation will be allowed.
- •Pregnancy or breast-feeding.
- •Uncontrolled diabetes mellitus (fasting blood glucose > 126 mg/dL or random blood glucose levels > 200 mg/dL on at least two occasions within 6 months prior to study entry).
- •Major organ transplantation.
- •Active alcohol or substance abuse or psychiatric illness, which in the opinion of the investigator will interfere with adherence to study requirements.
- •Clinically significant neurological disorder occurring within 1 year prior to study entry that in the opinion of the principal investigator would affect the subject's study compliance or safety.
- •Use of systemic corticosteroids for ≥ 4 weeks within 3 months prior to study entry.
- •Presence of any chronic disease that in the opinion of the investigator might affect subject safety.
- •History of previous vaccination with an HIV-1 vaccine.
- •History or evidence of autoimmune disease, including, but not limited to thyroid autoimmune disease and idiopathic thrombocytopenic purpura.
- •Allergies to bupivacaine or similar anesthetic.
结局指标
主要结局
Frequency of adverse events in each of the treatment arms
时间窗: Time of each vaccination, 2 weeks after vaccinations 3 and 4 and at the completion of the study.
次要结局
- Antigen specific cellular and humoral immune response.(2 weeks following the third and fourth vaccinations)
研究者
研究点 (2)
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