Nutritional Metabolomics: the Search for Dietary Exposure Variables

Not Applicable

Completed

• Conditions: Metabolic Disorders; Nutrition Disorders
• Interventions: Other: Diet

• Registration Number: NCT02039596
• Lead Sponsor: Göteborg University

Brief Summary

In the post-genomic era, a major challenge for health research is to understand the complex interactions among genetic, environmental and lifestyle factors including dietary intake. Unfortunately, such initiatives are hampered by the lack of accurate dietary intake assessment methods for large studies. The newly emerging field of metabolomics offers unique possibilities to characterize individual food intake, dietary patterns and effects of dietary intervention in large studies. The investigators propose to develop a platform to detect broad metabolomic responses to food intake in controlled trials as well as to use targeted metabolomics approaches to characterize dietary intake in longitudinal studies. Our laboratory has a long history of developing methodology for assessing nutritional status and effects of diet on metabolism. Here, the investigators team up with the Sahlgrenska Academy Core Facility and the Swedish NMR Centre at the University of Gothenburg, that offer modern metabolomics equipment and competence in bioinformatics, and use this in the context of nutrition research. To their knowledge, the investigators are among the first groups in the country to develop skills in metabolomics to assess dietary intake and effects of nutrition on metabolism, and probably the first to use two complementary platforms with both mass-spectrometry and nuclear magnetic spectroscopy. Hence, our methodological results should be useful to nutritional scientists nationally as well as internationally.

Detailed Description

Not available

Study Timeline

• Study Start: 2012-09
• Primary Completion: 2013-11
• Study Completion: 2013-11
• Study First Submitted: 2013-12-17
• Status Verified: 2014-01
• Study First Submitted QC: 2014-01-16
• Last Update Submitted: 2014-01-16
• Study First Posted: 2014-01-17
• Last Update Posted: 2014-01-17

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 53

Inclusion Criteria

• Body mass index 18.5-30.0
• Age 18-65 years old

Exclusion Criteria

• Medications or tobacco use

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
Swedish Breakfast	Diet	Diet: Swedish food items
English breakfast	Diet	Diet: English food items
vegetarian breakfast	Diet	Diet: Vegetarian food items
Lacto-vegetarian breakfast	Diet	Diet: Lacto-vegetarian food items
Swedish omnivore breakfast	Diet	Diet: Swedish breakfast with meat items

Primary Outcome Measures

Name	Time	Method
Acute metabolomic response	Directly after ingestion of test meal	The acute metabolomic response (ie all metabolites) to a test meal will be analyzed using both nuclear magnetic resonance (NMR) and mass spectrometry (MS) techniques. Patterns of metabolites will be evaluated with statistical techniques, ie discriminant analysis and principal component analysis.

Trial Locations

Locations (1)

Dept Internal Medicine and Clinical Nutrition, Univ of Gothenburg; 🇸🇪 Gothenburg, Sweden