Insect Protein - a New and Sustainable Protein Source to Maintain and Build Muscle
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 50
- 试验地点
- 2
- 主要终点
- Expression and phosphorylation of signaling proteins and their mRNA by Western Blotting and PCR
研究概览
简要总结
Randomized parallel study investing the effect of intake of different protein sources (whey, insect and pea) on the muscle protein synthesis. Activation of the signaling pathway leading to muscle protein synthesis is investigated by western blotting and Real time quantitative polymerase chain reaction (qPCR or PCR). Urine, blood and muscle is moreover investigated by metabolomics analysis.
详细描述
Background:
Sarcopenia is a syndrome highly prevalent in the older population, characterized by muscle loss and a decrease in muscle strength. This leads to loss of physical function, decreased life quality and well-being and an increased risk of early death. Research has shown that both ingestion of protein and physical activity is able to diminish the loss of muscle mass during aging and thereby reduce the prevalence of sarcopenia and diminish the consequence of the disease. This study will investigate if more sustainable protein sources of good quality can increase the muscle protein synthesis and thereby prevent sarcopenia.
Aim:
The aim of this project is to investigate the effects of insect protein on the stimulation of muscle protein synthesis. The effects of protein will both be studied alone and in combination with exercise in perspective of preventing loss of both muscle mass and muscle strength. The potential of insect protein will be elucidated by comparison with other alternative plant-based protein sources in this case pea protein. In addition, whey protein will be included as a positive control as it may be regarded as the most established dietary protein source combatting muscle loss.
Method:
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Prevention
- 盲法
- Double (Participant, Care Provider)
盲法说明
The project leader are responsible for the masking while all other persons involved in the project do not know the protein source given.
入排标准
- 年龄范围
- 18 Years 至 30 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •18-30 year
- •able to read and understand danish
排除标准
- •resistance training more than 1 time pr month
- •endurance training more than 2 hours pr week
- •Allergy toward shellfish, house dust mites or the protein sources.
- •knee problems effecting the exercise
- •weight change more than 5 kg the last half year
- •medicine which can effect the protein turnover
- •Disease in joint, or muscle as well as metabolic diseases.
结局指标
主要结局
Expression and phosphorylation of signaling proteins and their mRNA by Western Blotting and PCR
时间窗: 4 hours
Change in expression and phosphorylation of signaling proteins and the mRNA related the muscle protein synthesis.
Blod and urine metabolism by NMR
时间窗: 4 hours
Both blod and urine are analysed by nuclear magnetic resonance (NMR) metabolomics to enlighten changes in the metabolites.
Muscle metabolism by NMR
时间窗: 3 hours
The muscle metabolites are quantified before intervention, after protein and after protein end exercise.
次要结局
- BMI(1 hour)
- General physical activity(3 months)
- Blood Insulin(4 hours)
- Food intake, calories and distribution of macro nutrients.(1 day)
- Blood glucose(4 hours)