Effect of Smoking on the Expression of Gelatinases in Chronic Periodontitis

Completed

• Conditions: Periodontitis; Smoking
• Interventions: Other: no intervention

• Registration Number: NCT03426241
• Lead Sponsor: Baskent University

Brief Summary

This study comparatively evaluate the expressions of MMP-2 and MMP-9 in smoker vs non-smoker chronic periodontitis patients as well as periodontally healthy subjects. the hypothesis of this study was that smoking may interfere with periodontal disease by effecting MMP-2 and MMP-9 expressions.

Detailed Description

Smokers have greater risk for developing periodontal disease and smoking is highly correlated with greater marginal bone loss. Matrix metalloproteinases (MMP) play a major role in periodontal tissue destruction. MMP-2 and MMP-9, called gelatinases, are one of the major MMPs that plays important roles in periodontitis. To date, several studies showed the increased levels of gelatinases in periodontal disease and only a limited number of study investigated the effect of smoking on gelatinases. However, no previous study has directly investigated the possible relationship between smoking and gingival tissue gelatinases expressions (MMP-2 and MMP-9) in chronic periodontitis (CP) patients relative to periodontally healthy subjects.

Therefore this study is conducted to comparatively evaluate the expressions of MMP-2 and MMP-9 in smoker vs non-smoker chronic periodontitis patients as well as periodontally healthy subjects. the of this study hypothesis was that smoking may interfere with periodontal disease by effecting MMP-2 and MMP-9 expressions.

Study Timeline

• Study Start: 2010-02-10
• Primary Completion: 2012-12-29
• Study Completion: 2012-12-30
• Study First Submitted: 2018-01-29
• Status Verified: 2018-02
• Study First Submitted QC: 2018-02-07
• Last Update Submitted: 2018-02-07
• Study First Posted: 2018-02-08
• Last Update Posted: 2018-02-08

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 80

Inclusion Criteria

• A total of 80 subjects (35 females, 45 males, with an age range of 16-62 years) were included in this study from those patients seeking dental treatment at the Baskent University School of Dentistry between February 2010 and December 2012.

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Exclusion Criteria

• Patients with systemic disease;
• pregnant and/or lactating women;
• patients taking any medications that may influence the study.

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Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
smoking healthy	no intervention	-
smoking chronic periodontitis	no intervention	-
non-smoking periodontitis	no intervention	-
non-smoking healthy	no intervention	-

Primary Outcome Measures

Name	Time	Method
expression of gelatinases levels in gingival tissue among groups	day 1	In chronic periodontitis group, after recording the clinical parameters, gingival tissues were harvested from the deepest periodontal pocket. Whereas in periodontally healthy group, gingival tissues were collected during crown-lengthening surgery or during an extraction of a teeth for orthodontic reasons. In both groups the samples include both sulcus/pocket epithelium and underlying connective tissues. The expression of MMP-2 and MMP-9 is measured immunohistochemically

Secondary Outcome Measures

Name	Time	Method
difference in probing depths among groups	day 0	Clinical parameters will be recorded and difference in clinical attahment level among the groups will be calculated