NCT01397136
Completed
Not Applicable
Cell-free DNA: a Non-invasive Test for Assessing Embryo Quality
IRCCS Azienda Ospedaliera Universitaria San Martino - IST Istituto Nazionale per la Ricerca sul Cancro, Genoa, Italy1 site in 1 country600 target enrollmentJuly 2011
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Culture Media
- Sponsor
- IRCCS Azienda Ospedaliera Universitaria San Martino - IST Istituto Nazionale per la Ricerca sul Cancro, Genoa, Italy
- Enrollment
- 600
- Locations
- 1
- Primary Endpoint
- Isolation and characterization of nuclear and/or mitocondrial DNA into embryo culture medium
- Status
- Completed
- Last Updated
- 11 years ago
Overview
Brief Summary
In the present project we propose to deeply characterize nuclear and/or mitochondrial DNA in embryo culture medium, in order to investigate whether both cell-free nucleic acid types may provide additional, reliable, predictive parameters for embryo viability and implantation potential.
Investigators
Paola Scaruffi
Dr
IRCCS Azienda Ospedaliera Universitaria San Martino - IST Istituto Nazionale per la Ricerca sul Cancro, Genoa, Italy
Eligibility Criteria
Inclusion Criteria
- •We will enroll in the study only embryos obtained from fresh gametes. It is to note that we currently transfer more than one embryo (with a maximum of 3, according to patient's age) and we will follow such criteria alongside the project. Consequently, we will take into consideration this aspect in correlating DNA parameters with embryo's outcome.
Exclusion Criteria
- •We will exclude cryopreserved embryos, in order to avoid potential biases due to cooling/defrosting procedures.
Outcomes
Primary Outcomes
Isolation and characterization of nuclear and/or mitocondrial DNA into embryo culture medium
Time Frame: Month 6 of the project
Secondary Outcomes
- Prediction of high quality embryos according to molecular profiling of cell-free DNA released into culture medium.(Month 12 of the project)
- Publication of scientific reports.(Month 12 of the project)
- Dissemination of results by participating at national and international meetings, workshops and specific courses.(Months 3-12 of the project)
Study Sites (1)
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