A Controlled, Cross-over, Acute Intervention Study Investigating the Cognitive and Neuronal Effects of Flavonoids in Blueberries.
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 47
- 试验地点
- 1
- 主要终点
- Cognitive function
研究概览
简要总结
This study was a controlled, cross-over, acute flavonoid intervention trial with younger and older adults. Subjects consumed a blueberry beverage during one visit and a control beverage on another. Cognitive function pre drink was assessed, blood and urine samples were taken as well as blood pressure and a measure of vascular reactivity. These outcome measures were taken at 2 and 5 hours post drink.
It was predicted that the flavonoids in the blueberry drink would lead to improved performance on the cognitive tests and vascular reactivity measure compared to following the control drink. It was thought this could be due to increased vaso-dilation and improving blood flow to the brain which was investigated in an extension to the project where a sample of individuals underwent brain imaging in an MRI scanner pre and post a blueberry and a control drink.
详细描述
The control drink was matched to the blueberry drink for other bioactive compounds which may have affected cognition, specifically sugars and vitamin C. Volunteers were healthy, not on any medication, without high blood pressure, high cholesterol, high BMI, diabetes or other medical conditions. Older adults were aged 61-75 years and younger adults 18-26 years.
Blood and urine samples will be analysed for flavonoid levels and Brain Derived Neurotrophic Factor, a biomarker of memory and learning, flavonoids may lead to increased BDNF production through the ERK-CREB-BDNF pathway.
Flavonoids may also increase nitric oxide production and improve the flexibility of the blood vessels hence the measure of vascular reactivity using the Digital Volume Pulse machine. This can lead to increased vaso-dilation and blood flow to the brain, therefore an fMRI study was carried out the investigate this using arterial spin labeling following acute blueberry supplementation compared to a control drink.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- Single (Participant)
入排标准
- 年龄范围
- 18 Years 至 75 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •No medical conditions
- •Not taking any medication or supplements (or willing to stop taking supplements for duration of study)
- •Not lactose intolerant
- •Willing to give blood and urine samples
- •Not partaking in frequent vigorous exercise
- •Not suffering from or history of depression
排除标准
- •On blood pressure medication, taking Aspirin or other blood thinning medication
- •BMI > 30
- •Cholesterol > 6
- •Diabetes or other serious medical condition
- •Lactose intolerant
- •Any learning difficulty e.g. dyslexia
研究组 & 干预措施
Blueberry drink
30g of blueberry powder (equivalent to 200g fresh blueberries) and 300ml of semi-skimmed milk
干预措施: Flavonoids (Dietary Supplement)
Control drink
29g of powder consisting of sugars and vitamin C, values of which were matched to that of the blueberry drink, with 1 g of citric acid to match for taste.
干预措施: Control (Dietary Supplement)
结局指标
主要结局
Cognitive function
时间窗: pre drink, 2 hours and 5 hours post drink
Extensive cognitive test battery including tasks measuring executive function such as updating, and memory tests such as free recall.
次要结局
- Bioavailability and pharmacology(Pre drink and 1 hour post drink)
- Vascular Reactivity(Pre and 1 hour post drink)
- Neuronal effects(Pre and 1 hours post drink)
研究者
Jeremy Paul Edward Spencer
Professor of Nutritional Medicine
University of Reading