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Efficacy and Safety Study of Recombinant Human Arginase 1 in Patients With Relapsed or Refractory Acute Myeloid Leukemia

Phase 2
Conditions
Acute Myeloid Leukemia
Interventions
Biological: PEG-BCT-100
Registration Number
NCT02899286
Lead Sponsor
Bio-Cancer Treatment International Limited
Brief Summary

To evaluate the efficacy of PEG-BCT-100 in patients with relapsed or refractory acute myeloid leukemia (AML) in terms of remission rate.

Detailed Description

This is a phase 2, non-randomised, open-label study that aims at evaluating the efficacy of single agent PEG-BCT-100 in adult patients with relapsed/refractory AML. Eligible patients will receive intravenous (IV) infusion of PEG-BCT-100 weekly until disease progression, unacceptable drug-related toxicity(ies), allogeneic haematopoietic stem cell transplantation or withdrawal of subject consent.

Pharmacokinetic (PK) of PEG-BCT-100 and pharmacodynamics (PD) activity of PEG-BCT-100 on arginine depletion will be evaluated throughout the study. Plasma arginine level, intracellular blast arginine level (IBAL) in peripheral blood (PB) and bone marrow (BM) will be measured at specific time points. PEG-BCT-100 will be given once weekly at 1600 Units/kg (2.7mg/kg) per dose for three weeks (Cycle 1). If the post-treatment IBAL-BM examined within 5 days prior to each cycle fails to drop at least 70% from baseline value and disease response fails to achieve complete remission (CR) or complete remission with incomplete blood count recovery (CRi), PEG-BCT-100 may be increased to 2500 U/kg (the maximum tolerated dose as reported previously) at investigator's discretion. Disease response will be assessed within 5 days prior to each cycle according to the International Working Group (IWG) AML Response Criteria.

Safety and toxicity will be assessed through physical examinations, vital signs, blood tests and urinalysis throughout the study. Adverse event (AE) and serious adverse events (SAE) will be reported according to the National Cancer Institute Common Toxicity Criteria for Adverse Events version 4.03 (CTCAE v4.03) until 28 days after the last dose of PEG-BCT-100.

Immunogenicity response including anti-drug antibody (ADA) level and neutralizing antibody level will be assessed weekly for the first 2 cycles of PEG-BCT-100, pre-dose of each cycle thereafter and End of Study (EoS). Specific response predictive biomarkers in circulating and BM blasts, and emerging genetic markers will also be explored in the study.

Recruitment & Eligibility

Status
UNKNOWN
Sex
All
Target Recruitment
25
Inclusion Criteria
  1. Adult patients ≥18 year-old at the time of informed consent
  2. Documented relapsed or refractory AML after at least two standard chemotherapy regimen or in whom the treating physicians considered unfit for further chemotherapy treatment
  3. The Eastern Cooperative Oncology Group (ECOG) performance status equal or less than 2
  4. Patients from whom valid consent is obtained
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Exclusion Criteria
  1. Patients who have received any induction chemotherapy, investigational treatment and arginine depleting agent within 2 weeks prior to the start of the PEG-BCT-100 (not including hydroxyurea or thioguanine)
  2. Any toxic effects (except hair loss) of the prior therapy have not been resolved to Grade 1 or less according to National Cancer Institute Common Terminology Criteria for Adverse Events
  3. Total bilirubin > 1.5 x Upper Limit of Normal (ULN) not related to haemolysis or Gilbert's disease, and ratio of concentrations of aspartate transaminase and alanine transaminase (AST/ALT) > 5 x ULN
  4. Creatinine > 2 x ULN or estimated glomerular filtration rate using Modification of Diet in Renal Disease formula < 60 ml/min/1.73 m2
  5. Second active malignancy within the past year except basal cell or squamous cell skin cancer or carcinoma in situ of the cervix or breast
  6. Uncontrolled concomitant illness including, but not limited to symptomatic congestive heart failure, unstable angina pectoris or cardiac arrhythmia
  7. History of HIV-1 seropositivity
  8. Active infection not adequately responding to appropriate therapy
  9. Patient is pregnant or lactating
  10. Female with childbearing potential who is not willing to use contraceptive methods which, in the opinion of the investigator, are effective and adequate while on study treatment and for 6 months after the last dose of study treatment
  11. Male with a female partner with childbearing potential who is not willing to use contraceptive methods which, in the opinion of the investigator, are effective and adequate while on study treatment and for 6 months after the last dose of study treatment
  12. Any condition that is unstable or can jeopardize the safety of the patients and their compliance to the study
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Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
PEG-BCT-100PEG-BCT-100PEG-BCT-100 (PEGylated recombinant human arginase)
Primary Outcome Measures
NameTimeMethod
Complete remission (CR) rate3 years
Secondary Outcome Measures
NameTimeMethod
Time to progression (TTP)3 years
Overall survival (OS)3 years
PK - volume of distribution2 years
Anti-drug antibody (ADA)2 years

amount of ADA in patient sample (ng/mL)

Overall response rate (ORR)3 years

proportion of patients achieving CR or CRi or partial remission (PR)

Duration of remission3 years
Progression-free survival (PFS)3 years
PD2 years

arginine depletion

PK/PD relationship2 years

dose response

neutralizing anti-drug antibody (nADA)2 years

amount of nADA in patient sample (ng/mL)

AE and SAE3 years

Incidence of AE and SAE by severity grading as assessed according to CTCAE v4.03

PK - Area under the plasma concentration versus time curve (AUC)2 years
PK - Lowest concentration that PEG-BCT-100 reaches before the next dose is administered (Cmin)2 years
PK - elimination half-life2 years
PK - Peak plasma concentration of PEG-BCT-100 after administration (Cmax)2 years
PK - clearance2 years

Trial Locations

Locations (1)

The University of Hong Kong, Queen Mary Hospital

🇭🇰

Hong Kong, Hong Kong

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