Comparison of in Vitro Fertilization Rates of Oocytes and Embryo Development Using Two Techniques of Semen Processing

Not Applicable

Completed

• Conditions: Infertility, Female
• Interventions: Other: SSA sperm selection

• Registration Number: NCT03186170
• Lead Sponsor: University of Campinas, Brazil

Brief Summary

The investigators propose to assess one method of sperm selection based on the characteristics of spermatozoa recently introduced: Sperm Selection Assay, which used a gradient of concentration of progesterone to act as chemoatractant of spermatozoa of better quality. We will assess in vitro fertilization with two techniques (SSSA versus traditional technique of swin up) as well as embryo development.

Detailed Description

The project is a RCT follow the orientation of CONSORT. The investigators propose to assess one method of sperm selection based on the characteristics of spermatozoa recently introduced: Sperm Selection Assay, which used a gradient of concentration of progesterone to act as chemoatractant of spermatozoa of better quality. We will assess in vitro fertilization with two techniques (SSSA versus traditional technique of swin up) as well as embryo development.

Study Timeline

• Study First Submitted: 2017-05-22
• Study Start: 2017-06-01
• Study First Submitted QC: 2017-06-12
• Study First Posted: 2017-06-14
• Status Verified: 2017-07
• Primary Completion: 2017-07-15
• Study Completion: 2017-07-15
• Last Update Submitted: 2017-07-23
• Last Update Posted: 2017-07-25

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Female
• Target Recruitment: 30

Inclusion Criteria

• infertile women who will partcipate in an IVF programme

Exclusion Criteria

• male factor of infertility

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
control	SSA sperm selection	The investigators propose to assess one method of sperm selection based on the characteristics of spermatozoa recently introduced: Sperm Selection Assay, which used a gradient of concentration of progesterone to act as chemoatractant of spermatozoa of better quality. We will assess in vitro fertilization with two techniques (SSSA versus traditional technique of swin up for sperm selection) as well as embryo development. In this arm, the sperm selection for IVF will be the traditional swin up technique with different Percoll gradient Sperm selection via traditional swin-up
SSA	SSA sperm selection	The investigators propose to assess one method of sperm selection based on the characteristics of spermatozoa recently introduced: Sperm Selection Assay, which used a gradient of concentration of progesterone to act as chemoatractant of spermatozoa of better quality. We will assess in vitro fertilization with two techniques (SSA versus traditional technique of swin up) as well as embryo development. In this arm, the sperm selection for IVF will be performed by the new proposed technique of Sperm Selection Assay which consist to expose spermatozoa a progesterone quemoatractant Sperm selection via SSA technique

Primary Outcome Measures

Name	Time	Method
In vitro fertilization: Rate of embryo fertilizated	two weeks	we will asses the rate of embryo fertilizated

Secondary Outcome Measures

Name	Time	Method
Embryo development after IVF: Rate of embryo development	two weeks	we will asses the rate of embryo development

Trial Locations

Locations (1)

University of Campinas; 🇧🇷 Campinas, SP, Brazil