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Is Lymphocyte Subtype Important for Acute Pancreatitis Severity?

Completed
Conditions
Immune System Diseases
Lymphocytic Infiltrate
Pancreatitis
Interventions
Diagnostic Test: Flow Cytometric analysis
Registration Number
NCT04502940
Lead Sponsor
Istanbul Training and Research Hospital
Brief Summary

Pancreatitis is a common complication especially in patients with gallbladder stones, most patients with biliary pancreatitis may recover spontaneously without sequelae, but in 10-20% of patients, the disease is severe and mortality rates of up to 30% are detected in these patients. In the evaluation of acute biliary pancreatitis, many scoring systems have been established (Atlanta, Ranson, APACHE, BISAP etc.) from past to present to determine morbidity and mortality of the disease.

In this study, the investigators aimed to evaluate the correlation between morbidity and mortality of acute biliary pancreatitis and lymphocyte subtypes with Flow-cytometry.

Detailed Description

Pancreatitis is a common complication especially in patients with gallbladder stones, most patients with biliary pancreatitis may recover spontaneously without sequelae, but in 10-20% of patients, the disease is severe and mortality rates of up to 30% are detected in these patients. In patients with severe pancreatitis, aggressive fluid replacement, organ damage follow-up, appropriate antibiotherapy, and endoscopic sphincterotomy and radiological interventions may be of great benefit. In the evaluation of acute biliary pancreatitis, many scoring systems have been established (Atlanta, Ranson, APACHE, BISAP etc.) from past to present to determine morbidity and mortality of the disease. There are limited number of studies in the literature about the immune parameters in the evaluation of acute pancreatitis. In a studies, serum inflammatory markers such as IL-1, IL-6 and CD4, CD8 T lymphocyte and Treg population were evaluated.Treg cells are reported to be an independent prognostic factor in determining the severity of acute pancreatitis. In patients diagnosed with acute biliary pancreatitis, determination of the course of the disease at the time of diagnosis is extremely important for treatment and survival.

In this study, the investigators aimed to evaluate the correlation between morbidity and mortality of acute biliary pancreatitis with lymphocyte subtypes via Flow-cytometry.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
53
Inclusion Criteria
  • 18-80 years old
  • Diagnosis of acute biliary pancreatitis
  • Sign the voluntary consent form
Exclusion Criteria
  • Being under 18 or older than 80
  • Not signing the voluntary consent form
  • Pancreatitis resulting from an interventional procedure (ERCP, surgery, etc.)
  • Pregnant women
  • Being a history of immunodeficiency
  • Cancer history

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Arm && Interventions
GroupInterventionDescription
Mild pancreatitisFlow Cytometric analysisPatients with mild acute biliary pancreatitis according to Atlanta classification
Moderate PancreatitisFlow Cytometric analysisPatients with moderate acute biliary pancreatitis according to Atlanta classification
Severe pancreatitisFlow Cytometric analysisPatients with severe acute biliary pancreatitis according to Atlanta classification
ControlFlow Cytometric analysisHealthy volunteers
Primary Outcome Measures
NameTimeMethod
Lymphocyte subtypes 19 months

T helper (CD4) (%)

Lymphocyte subtypes 39 months

Treg (CD25 and CD127) (%)

Lymphocyte subtypes 29 months

T cytotoxic (CD8) (%)

Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (2)

İstanbul Training and Research Hospital

🇹🇷

Istanbul, Turkey

Gaziosmanpaşa Taksim Eğitim ve Araştırma Hastanesi

🇹🇷

Istanbul, Turkey

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