The Pathogenesis of Human Senile Cataract

• Conditions: Cataract
• Interventions: Procedure: phacoemulsification

• Registration Number: NCT03907358
• Lead Sponsor: Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine

Brief Summary

To study the pathological mechanism of cataract by genomics, transcriptomics, and proteomics.

Detailed Description

Tissue samples and aqueous humor samples will be collected from each individual patients by cataract surgery. After sample treatment, different techniques will be applied for analysis. And bioinformatics approach is applied for data treatment and analysis.The data of genomics, transcriptomics, and proteomics will be analyzed for study of pathological mechanism of cataract.

Study Timeline

• Study Start: 2016-12-07
• Status Verified: 2019-04
• Study First Submitted: 2019-04-04
• Study First Submitted QC: 2019-04-06
• Last Update Submitted: 2019-04-06
• Study First Posted: 2019-04-09
• Last Update Posted: 2019-04-09
• Primary Completion: 2019-12-30
• Study Completion: 2020-06-30

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: All
• Target Recruitment: 20

Inclusion Criteria

• Patients with the explicit diagnosis of cataract (including senile cataract, congenital cataract, complicated cataract, metabolic cataract, traumatic cataract, etc.) in the operated eye are willing to undergo cataract surgery.

Exclusion Criteria

• Patients whose cataracts need not be operated
• Patients with any contraindications to cataract surgery
• Patients are unwilling to undergo cataract surgery

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
cataract in young age	phacoemulsification	-
cataract in old age	phacoemulsification	-

Primary Outcome Measures

Name	Time	Method
Characterization of miRNA in micro-scale human aqueous humor	12 month	method development for miRNA detection in aqueous humor
Study of cataract by genomics, transcriptomics, and proteomics approaches	36 month	detect histone H3 lysine 4 trimethylation (H3K4me3) of aqueous humor of cataract patients by genomics, transcriptomics, and proteomics methods
Favored amplification recovery via protection-chromatin immunoprecipitation-Seq of human eye tissue samples	24 month	low-cell-number epigenome profiling for study of cataract

Trial Locations

Locations (1)

Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University; 🇨🇳 Shanghai, China