Lateral Ramus Cortical Bone Plate in Alveolar Cleft Osteoplasty: Concomitant Use of Buccal Fat Pad Derived Cells and Autogenous Bone in a Preliminary Study
Overview
- Phase
- Phase 1
- Intervention
- Not specified
- Conditions
- Cleft of Alveolar Ridge
- Sponsor
- Shahid Beheshti University of Medical Sciences
- Enrollment
- 10
- Locations
- 1
- Primary Endpoint
- change in bone volume
- Status
- Completed
- Last Updated
- 9 years ago
Overview
Brief Summary
Our aim was to combine regenerative techniques with bone grafting in human models to increase predictability and survival of reconstructed tissue. The MSCs in this study were derived from an intra oral fat source (BFP) and were cultured over natural bovine bone mineral granules and delivered within the lateral ramus cortical bone plate (LRCP)to treat human alveolar cleft defects.
Detailed Description
Patients enrolled in the present study were suffering from alveolar cleft and had previously received orthodontic treatment and only required secondary alveolar grafting. The buccal fat pad derived stem cells (BFPSCs) were harvested from buccal fat pad tissues of the patients. Patients were divided into 3 groups. Group A , the control group, was treated with anterior iliac crest (AIC) spongy bone to fill defects, followed by coverage with collagen membrane. Group B was treated with lateral ramus cortical bone cage (LRCP), used to create a protected healing space by fixing it to adjacent walls of the cleft defect. Group C was treated with (AIC) as in Group 1, but BFP-derived MSCs cultured over NBBM were put over the spongy bone and covered with a collagen membrane.Passage 3 BFPSCs were loaded on the implants ( Cerabone (Botiss, Berlin, Germany) a natural bovine bone mineral (NBBM); a granular biomaterial with a 200- to 850-µm particle size) 3 days prior to transplantation.Cone beam computed tomography (CBCT) was obtained after 6 months.
Investigators
Arash Khojasteh
Associate professor
Shahid Beheshti University of Medical Sciences
Eligibility Criteria
Inclusion Criteria
- •Unilateral congenital cleft lip and palate
- •Previous presurgical orthodontic treatment
Exclusion Criteria
- •History of malignancy
- •History of radiation
- •History of chemotherapy
- •Pregnancy
- •Systemic diseases contradicting dental and surgical treatments
- •Conditions or drugs affecting bone remodeling or bone metabolism and connective tissue
- •Allergy to collagen
Outcomes
Primary Outcomes
change in bone volume
Time Frame: before the surgery and 6 months after the surgery
1-mm coronal sections of the treated region were taken before and after surgery, and new bone formation was assessed by CBCT.