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Evaluation of PSMA in HER2- AR+ Metastatic Breast Cancer

Phase 2
Recruiting
Conditions
Breast Cancer
HER2-negative Breast Cancer
Metastatic Breast Cancer
Interventions
Registration Number
NCT04573231
Lead Sponsor
University of Wisconsin, Madison
Brief Summary

The purpose of this research is to determine the expression of prostate specific membrane antigen (PSMA) in human epidermal growth factor receptor 2 (HER2)-negative, androgen receptor (AR)-positive metastatic breast cancer, and to determine its role in resistance to the anti-androgen, bicalutamide. The investigators hypothesize that PSMA expression will correlate with resistance to anti-androgen therapies, as has been documented in prostate cancer, and this can be used to select patients most likely to benefit from these therapies in future clinical trials. 15 people with HER2-negative, AR-positive metastatic breast cancer will be enrolled and be on study for about 3 days.

Detailed Description

Primary Objective:

* To evaluate the expression of PSMA via 18F-DCFPyL PSMA-based PET/CT in patients with metastatic HER2-negative, AR-positive breast cancer. Expression of PSMA will be quantified using PSMA-based PET imaging using a novel agent, 18F-DCFPyL, as a non-invasive imaging biomarker of tumor neovasculature in HER2-negative, AR-positive metastatic breast cancer.

Secondary Objectives:

* PSMA PET will be compared with the expression of PSMA in CTCs and diagnostic metastatic tissue from patients with HER2-negative, AR-positive metastatic breast cancer.

* PSMA expression will be correlated with clinical benefit (objective response and progression-free survival) to bicalutamide and ribociclib for patients enrolled in NCT03090165.

Recruitment & Eligibility

Status
RECRUITING
Sex
All
Target Recruitment
13
Inclusion Criteria
  • Patients diagnosed with metastatic HER2-negative breast cancer AR expression of ≥ 10%
Exclusion Criteria
  • Other (non-breast) known active malignancy. Participants with previously treated cancers which are in remission or have no evidence of disease are eligible.
  • Unable to lie flat during or tolerate PET/CT
  • Participants with any medical condition or other circumstances that, in the opinion of the investigator, compromise the safety or compliance of the subject to produce reliable data or completing the study
  • Women of childbearing potential must not be pregnant or breast feeding (pregnancy test negative within 7 days prior to PET/CT

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
18F-DCFPyL PSMA-based PET/CT18F-DCFPyL* 18F-DCFPyL whole body PET/CT scan * Review of relevant imaging and medical record information * Blood draw for circulating tumor cells (CTCs) * Analysis of diagnostic tissue specimens
Primary Outcome Measures
NameTimeMethod
PSMA Expression as measured by 18F-DCFPyL SUVup to 3 days

Quantitative analysis of 18F-DCFPyL PSMA PET uptake will be performed at the lesion-level. Standardized uptake values (SUV) will be measured by manually drawing a region of interest (ROI) to encompass the entire lesion guided by the lesion extent visualized on conventional imaging. PET SUV based quantitative tumor uptake parameters will be obtained.

Secondary Outcome Measures
NameTimeMethod
Expression of PSMA in diagnostic metastatic tissueup to 3 days

PSMA expression in tumors will be semi-quantitatively scored by a study pathologist using published methods including separate assessment of tumor-associated and non-tumor vasculature, with the endothelium highlighted by a CD31 immunostain to facilitate definitive recognition.

Change in PSMA Expression on CTCs after 2 weeks of bicalutamidebaseline and up to 2 weeks

The change in PSMA expression on CTCs will be measured pre and post treatment via immunofluorescence.

Expression of PSMA in CTCsup to 2 weeks

Expression of PSMA in CTCs will be measured via immunofluorescence.

Trial Locations

Locations (1)

University of Wisconsin School of Medicine and Public Health

🇺🇸

Madison, Wisconsin, United States

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