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Trial of PXD101 (Belinostat) in Combination With Idarubicin to Treat AML Not Suitable for Standard Intensive Therapy

Phase 1
Completed
Conditions
Acute Myeloid Leukemia
Interventions
Registration Number
NCT00878722
Lead Sponsor
Valerio Therapeutics
Brief Summary

An open-label, non-randomized, multi-centre, Phase I/II trial to assess the efficacy and safety of 2 schedules of PXD101 in combination with idarubicin in patients with AML not suitable for standard intensive therapy.

Detailed Description

This trial is an open-label, multi-centre, dose-escalation Phase I/II study to evaluate safety, explore efficacy, pharmacodynamics, and pharmacokinetics of the combination of PXD101 with idarubicin administered in two different schedules in patients with AML. The PXD101 plus idarubicin treatment will be repeated at suitable intervals (target is every 3 weeks for schedule A and every 2 weeks for schedule B) depending upon toxicities or disease progression. Safety and efficacy assessments will be performed at every cycle.

Schedule A uses PXD101 by 30 min infusion daily for 5 days every 3 weeks with escalating doses of idarubicin.

Schedule B uses escalating doses of continuous infusion (48h) of PXD101 alone or in combination with idarubicin.

In both regimens the trial may be expanded at the Maximum Tolerated Dose (MTD).

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
41
Inclusion Criteria

(abbreviated)

  1. Signed consent

  2. AML patients:

    1. above 60 years in first relapse or refractory.
    2. 18-60 years 2nd relapse or refractory to at least two intensive chemotherapy regimens.
    3. above 60 years with high risk features (cytogenetics, secondary or treatment related AML) d) above 60 years with myelodysplastic syndrome with >10% blasts in bone marrow (WHO RAEB-2 (Refractory anemia with excess blasts-2)). For patients below 60 years potential curative treatments should have been exhausted.
  3. Performance status (ECOG) ≤ 2

  4. Age ≥ 18 years

  5. Acceptable liver, renal and bone marrow function as defined

  6. Serum potassium within normal range.

  7. Acceptable coagulation status as defined

  8. Precautions for female patients with reproductive potential as defined

Exclusion Criteria
  1. Treatment with investigational agents within the last 4 weeks
  2. Prior treatment with HDAC (Histone deacetylases) inhibitors including valproic acid
  3. Prior anti-leukemic therapy (except hydroxyurea) within the last 3 weeks of trial dosing
  4. Co-existing active infection (including HIV) or any co-existing medical condition likely to interfere with trial procedures, including significant cardiovascular disease
  5. Altered mental status precluding understanding of the informed consent process and/or completion of the necessary study procedures.
  6. Concurrent second malignancy.
  7. History of hypersensitivity to idarubicin
  8. Cumulative idarubicin dose exceeding 100 mg/m², or a (with respect cardiotoxicity) corresponding dose of other anthracyclines
  9. LVEF (left ventricular ejection fraction) below normal range (< 45% )
  10. Known Central Nervous System (CNS) leukemia

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
Arm BPXD101PXD101 administered by continuous intravenous infusion over 24-48 hours and idarubicin (in the later steps) added after the first 24 hours. The second cycle will start on day 15 but under observation of possible toxicity. Further cycles will be administered q 14 d for up to 6 cycles. The first dose steps will be carried out with PXD101 alone for safety reasons.
Arm AidarubicinPXD101 administered as a 30-minute intravenous (IV) infusion of 1000 mg/m²/d for five consecutive days every 3 weeks. Idarubicin administered on day 5 (first steps) or days 4 and 5 (later steps). Patients will be treated in a 21-day cycle for a minimum of 2 cycles and a maximum of 6 cycles (depending on cumulated idarubicin dose).
Arm BidarubicinPXD101 administered by continuous intravenous infusion over 24-48 hours and idarubicin (in the later steps) added after the first 24 hours. The second cycle will start on day 15 but under observation of possible toxicity. Further cycles will be administered q 14 d for up to 6 cycles. The first dose steps will be carried out with PXD101 alone for safety reasons.
Arm APXD101PXD101 administered as a 30-minute intravenous (IV) infusion of 1000 mg/m²/d for five consecutive days every 3 weeks. Idarubicin administered on day 5 (first steps) or days 4 and 5 (later steps). Patients will be treated in a 21-day cycle for a minimum of 2 cycles and a maximum of 6 cycles (depending on cumulated idarubicin dose).
Primary Outcome Measures
NameTimeMethod
Maximum Tolerated Dose, Dose Limiting ToxicityFirst Cycle

DLT (dose limiting toxicities): patients with any of the toxicities: 1.Haematological toxicity is not included in the definition due to bone marrow involvement by the disease except for following grade 4 ANC (absolute neutrophil count) and PLT (platelet count) for 6 weeks with less than 5% blasts in bone marrow. 2.Drug related non hematological Grade 3 or 4 toxicity except alopecia, brief nausea and vomiting, diarrhea, rash, arthralgias and myalgias. Treatment interventions should palliate toxicity symptoms prior to concluding a DLT has occurred (e.g if nausea and vomiting to Grade 3 have been associated with the drug). If despite standard treatment Grade 3 nausea and or vomiting persisted then a DLT was considered to have occurred. Grade 4 diarrhea in spite of standard therapeutic measures was included in DLT definition. 3.Inability to tolerate full dosing cycle due to toxicity or any drug-related adverse event resulting in more than 14 day treatment delay in the next treatment cycle

Overall ResponseThroughout study, after each cycle for the first two cycles, then after every second cycle

Efficacy measured as Response rate (complete response (\[CR\] and Complete remission with incomplete recovery of platelets \[CRi\]) and partial response (\[PR\])) using the response criteria of the International Working Group (Cheson et al 2003). CR includes CRi, CRc (Cytogenetic complete remission), and CRm (Molecular complete remission).

Secondary Outcome Measures
NameTimeMethod
Time to Response (CR and PR)Throughout study, after each cycle for the first two cycles, then after every second cycle

Time to response: time in weeks from first treatment to obtainment of the particular response status (CR and PR)

Duration of Response (CR and PR)Throughout study, after each cycle for the first two cycles, then after every second cycle

Duration of Response (CR and PR) in Weeks

Overall SurvivalThroughout study, after each cycle for the first two cycles, then after every second cycle

Overall survival: time in weeks from entry into study until death from any cause. All patients without this endpoint at the time of discontinuation or the end of trial have been censored.

Relapse-Free SurvivalThroughout study, after each cycle for the first two cycles, then after every second cycle

Relapse-free survival: time (weeks) from leukemia-free state to relapse or death from any cause.

Event-Free SurvivalThroughout study, after each cycle for the first two cycles, then after every second cycle

Event-free survival: time (weeks) from entry into study until treatment failure, disease relapse or death from any cause.

Remission DurationThroughout study, after each cycle for the first two cycles, then after every second cycle

Remission duration: time (weeks) from date of remission status to disease relapse.

Belinostat CmaxSamples taken in Cycle 1 only, prior to initial dose on days 4 and 5 and at end of infusion, 5, 15, and 30 min, and 1, 2, 3, 4, and 6 hours post infusion

Cmax: Arm A: at Cycle 1 Day 4, Cycle 1 Day 5 Arm B: Cycle 1 Day 1 and Cycle 1 Day 2

Belinostat AUC (Area Under Curve)Cycle 1, prior to initial dose on days 4 and 5 and at end of infusion, 5, 15, and 30 min, and 1, 2, 3, 4, and 6 hours post infusion
Elimination t½Cycle 1, Samples taken in Cycle 1 only, prior to initial dose on days 4 and 5 and at end of infusion, 5, 15, and 30 min, and 1, 2, 3, 4, and 6 hours post infusion

Trial Locations

Locations (6)

Hôpital St. Louis

🇫🇷

Paris, France

Uniklinik Homburg

🇩🇪

Homburg, Germany

Uni Hospital Marburg

🇩🇪

Marburg, Germany

Christie Hospital NHS Trust

🇬🇧

Manchester, United Kingdom

CHU Lapeyronie

🇫🇷

Montpellier, France

Universitätsklinikum Ulm

🇩🇪

Ulm, Germany

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