Influence of Endurance Training and Histamine Receptor Antagonists on the Transcriptome Response in Human Muscle
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 14
- 试验地点
- 2
- 主要终点
- Change in muscle transcriptome (direct response)
研究概览
简要总结
Blocking histamine H1/H2 receptors blunts chronic endurance training adaptations. The current study addresses a twofold research question: "What is the influence of endurance training (1) and histamine H1 and H2 signaling (2) on the gene expression in human skeletal muscle." Results from this study will yield more insights into the molecular mechanisms of adaptations to exercise training.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- Triple (Participant, Investigator, Outcomes Assessor)
入排标准
- 年龄范围
- 18 Years 至 45 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Male and female
- •18-45 years
- •not to medium physically active
排除标准
- •Chronic disease
- •Supplement or medication intake
- •Seasonal allergies
结局指标
主要结局
Change in muscle transcriptome (direct response)
时间窗: On each test-day: 0 minutes after the exercise training versus at rest.
RNA-sequencing
Change in muscle transcriptome (delayed response)
时间窗: On each test-day: 180 minutes after the exercise training versus at rest.
RNA-sequencing
Muscle glycogen resynthesis
时间窗: On each test-day: 180 minutes after the exercise training versus at rest.
Fluorometric determination of muscle glycogen levels
Plasma volume change
时间窗: Change from rest to different time-points after exercise (0, 30, 60, 120 and 180 minutes after the exercise training).
Plasma volume based on hemoglobin and hematocrit concentration.
Muscle glycogen depletion
时间窗: On each test-day: 0 minutes after the exercise training versus at rest.
Fluorometric determination of muscle glycogen levels
次要结局
- Heart rate during the exercise training(Continuously during training on each test-day.)
- Blood lactate(On each test-day at 10 time-points: at rest, during exercise and 0, 30, 60, 120 and 180 minutes after the exercise training.)
- Blood glucose(On each test-day at 10 time-points: at rest, during exercise and 0, 30, 60, 120 and 180 minutes after the exercise training.)
- Blood histamine(On each test-day at 6 time-points: at rest and 0, 30, 60, 120 and 180 minutes after the exercise training.)
- Blood insulin(On each test-day at 6 time-points: at rest and 0, 30, 60, 120 and 180 minutes after the exercise training.)
- Muscle signaling pathways relevant for glucose metabolism and cardiovascular health(On each test-day: at rest, 0 minutes and 180 minutes after the exercise training.)