Investıgatıon of The Effıciency of Pulsed Electromagnetıc Field Therapy and Stretching Exercises

Not Applicable

Completed

• Conditions: Pulsed Electromagnetic Therapy; Muscle Stretching Exercise; Muscle Injury
• Interventions: Device: Pulsed Electromagnetic Field Therapy; Other: Exercise

• Registration Number: NCT05988476
• Lead Sponsor: Suleyman Demirel University

Brief Summary

In this study, a total of 69 Wistar Albino rats were used, five of which were in the preliminary study. The preliminary study was planned to determine the ideal PEMF treatment time. In the study, animals were divided into 5 groups (Control, INJ, INJ+Exercise, INJ+PEMF, INJ+Exercise+PEMF). Experimental animals were sacrificed on the 7th and 14th days to see the effects of the treatments. At the end of the experiment, genetic, histopathological, and immunohistochemical evaluations were made in the muscle tissue.

Detailed Description

Purpose: The aim of this study was to evaluate the efficacy of stretching exercise and the pulsed electromagnetic field (PEMF) method in the treatment of muscle injury (INJ).

Methods: In this study, a total of 69 Wistar Albino rats were used, five of which were in the preliminary study. The preliminary study was planned to determine the ideal PEMF treatment time. In the study, animals were divided into 5 groups (Control, INJ, INJ+Exercise, INJ+PEMF, INJ+Exercise+PEMF). Experimental animals were sacrificed on the 7th and 14th days to see the effects of the treatments. At the end of the experiment, genetic, histopathological, and immunohistochemical evaluations were made in the muscle tissue.

Study Timeline

• Study Start: 2022-09-05
• Primary Completion: 2022-10-02
• Study Completion: 2022-11-07
• Status Verified: 2023-08
• Study First Submitted: 2023-08-04
• Study First Submitted QC: 2023-08-04
• Last Update Submitted: 2023-08-04
• Study First Posted: 2023-08-14
• Last Update Posted: 2023-08-14

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Female
• Target Recruitment: 64

Inclusion Criteria

• Healthy young wistar albino rats

Exclusion Criteria

• not having any injury

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
Stretching Exercise	Pulsed Electromagnetic Field Therapy	-
Pulsed Electromagnetic Field and Stretching Exercise	Exercise	-
Pulsed Electromagnetic Field and Stretching Exercise	Pulsed Electromagnetic Field Therapy	-
Pulsed Electromagnetic Field	Exercise	-

Primary Outcome Measures

Name	Time	Method
Mononuclear cell infiltration, Atrophy and necrosis, Pyknotic nucleus assesment at the end of two weeks	two week	hematoxylin-eosin staining
TGF-β1, eNOS assesment at the end of two weeks	two week	Quantitative Reverse-Transcriptase Polymerase Chain Reaction (RT-qPCR)
VEGF and FGF assesment at the end of two weeks	two week	immunohischemical staining

Trial Locations

Locations (1)

SuleymanDU; 🇹🇷 Isparta, Turkey