Blastocyst Culture in a Time-lapse Incubator
- Conditions
- Sub-optimal Culture Conditions
- Interventions
- Procedure: Culture media renwal
- Registration Number
- NCT02778217
- Lead Sponsor
- Dar AlMaraa Center
- Brief Summary
Culture media used to support development of zygotes to the blastocyst stage is based on two basic philosophies: a single step medium "let-the-embryo-choose" or a sequential medium "back-to-nature".
For single step media, there are two types of protocol can be used for the culture of human preimplantation embryos:
1. Interrupted single medium culture, in which the medium is renewed on the Day 3 of embryo culture
2. Uninterrupted single embryo culture, in which the same single medium is used throughout the 5-6 days of culture with no replenishment or replacement on Day 3.
Investigators aim to compare the blastocyst development and clinical outcome between the two types of protocols of a single medium (with or without renewal on Day 3)
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- UNKNOWN
- Sex
- Female
- Target Recruitment
- 200
- female partners were under the age of 39 years
- Intracytoplasmic sperm injection (ICSI) was performed using fresh ejaculates
- Day 3 embryo transfer cycles
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Arm && Interventions
Group Intervention Description Interrupted single medium culture Culture media renwal The single step medium is renewed on Day 3 of embryo culture.
- Primary Outcome Measures
Name Time Method Clinical pregnancy rate 4 weeks post embryo transfer diagnosed by the presence of a heartbeat in the gestational sac visualized by ultrasound
- Secondary Outcome Measures
Name Time Method Implantation rate 4-6 weeks post embryo transfer the number of intrauterine gestational sacs over the total number of embryos transferred
Blastocyst formation rate 5-6 days post intracytoplasmic sperm injection
Trial Locations
- Locations (1)
Sunrise fertility center
🇪🇬Cairo, Egypt