Relation Between Fatty Acids and Oxidative Stress Markers With Cardiovascular Risk Factors in a Healthy Population

Background Cardiovascular diseases belong to the major causes of mortality in western populations. Atherosclerosis of arterial system may result in ischemic heart disease (IHD) and peripheral artery disease with possible fatal consequences such as acute myocardial infarction and stroke.

Atherosclerosis is a chronic and slowly growing pathological lesion that remains asymptomatic until a clinical event occurs. In the pre-clinical stage atherosclerosis may be difficult to diagnose. As disease surrogate, a large number of risk factors for atherosclerosis are being recognized. Some of them are responsible for the epidemiologically very serious metabolic syndrome, which accounts for development of hyperlipidemia (from preventive point of view only 25% of population has normal blood lipid levels), obesity, diabetes or arterial hypertension. In all of these conditions, oxidative stress represents a substantial pathogenic factor through deleterious chemical reactions that damage biomolecules, including formation of atherogenic- oxidatively-modified LDL particles (1) or direct destruction of cells and tissues by free radicals (2-4). Lifestyle, in particular dietary schemes, belongs to important factors contributing to the formation and development of atherosclerosis. The usefulness of dietary components in contributing to low cardiovascular mortality in populations of the mediterranean basin is well recognized. After pioneering studies by Ancel Keys (5), Mediterranean diet has become popular worldwide as health diet. Several components of this diet have been advocated in contributing to counteract atherosclerosis, including a panel of antioxidants and unsaturated fatty acids. Health providers in continental diet-based countries suggest to embracing Mediterranean diet in order to contribute in reducing cardiovascular mortality. However, countries in the Mediterranean area are experiencing a shift in dietary habit towards continental diet with potential harmful change in mortality rates. Recently, Trichopoulou et al. (6) reported on adherence to mediterranean diet and survival in a Greek population. These authors found that adherence to this diet is associated with a significant reduction in mortality.

Italy is being challenged in lifestyle and dietary habit issues because such a drift has already caused an increase in obesity prevalence in children. Paradoxically, this is much higher in the south of Italy that is expected to be more strictly associated with mediterranean style.

It is not known, however, which components of the Mediterranean diet act protectively in the organism. It is suggested that certain components of the diet may importantly affect oxidative stress (7) and thereby also occurrence of cardiovascular (8) and tumor diseases (9). There are also very interesting data on the relation between regional dietary habits and diet composition, and the occurrence of cardiovascular diseases. From this point of view Mediterranean region seems to be exemplary. Low incidence of cardiovascular diseases was demonstrated here already in 50´s in spite of relatively high fat intake, whose major source was, however, olive oil rich in monounsaturated fatty acids and antioxidants such as polyphenolic compounds 12(10). Mediterranean diet was proved also in controlled studies to have marked protective effects on development of cardiovascular diseases (11, 12), but also total mortality (6) probably by action of antioxidant substances increasing total antioxidant capacity (13). The role of dietary factors on total antioxidant status was described in numerous studies, which showed marked increase of antioxidant potential after administration of vitamin A (14), vitamin C (15), tomatoes or tomato products containing high amounts of antioxidative terpen lycopene or lycopene together with olive oil (16).

On the other hand, it is emerging that previously unrecognized products of lipid metabolism may have profound effects on metabolic syndrome and potentially on the induction of diabetes. Palmitoleate has been recognized as an adipose-tissue derived lipid hormone that strongly stimulates muscle insulin action and suppresses hepatosteatosis (17). If palmitoleate acts as a lipid hormone in human is not known. In human, other fatty acids have been demonstrated to be altered in clinical conditions opposite to obesity. In fact, in cystic fibrosis patients, who are usually malnourished, we detected an increase in saturated and monounsaturated fatty acids (18) and a decrease in n-6 polyunsaturated fatty acids. C24:0 was positively correlated with BMI, plasma cholesterol and vitamin E, and significantly negatively correlated with oxysterols.Taken together, these data possibly identifies C24:0 as an indicator of adequate nutriture, coupled with the observation that C24:0 levels were reduced in patients with pancreatic insufficiency.