Secretion Induction of Neutrophil Extracellular Traps (NETs)in Inflammatory and Infectious Environment of Mucoviscidose
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 55
- 试验地点
- 1
- 主要终点
- the DNA concentration secreted into the extracellular environment neutrophil activated
研究概览
简要总结
The study is defined in 3 stages of in vitro assays :
Phase 1 : Secretion of NETs in response to lung disease and their secretion of factors.
This is a transversal study in which we compare :
- Producing NETs (DNA and proteases ) by neutrophils purified from sputum and blood of patients
- Producing NETs (DNA and proteases ) by purified neutrophils in the blood of patients and blood of control subjects
Phase 2: Mechanisms of activation of NETs secretory pathways . This phase will identify receptors (TLR ) involved in the secretion of NETs and analyze the involvement of dependent transduction pathways calcium and PKC . It will be performed on control blood neutrophils.
Phase 3: Analysis of cell death ( NETose )
This is a transversal study that will analyze :
the mode of cell death of neutrophils purified blood and sputum of patients and control blood neutrophils by flow cytometry and transmission electron microscopy .
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Men or women over 18
- •Patients with mucoviscidose in stable condition, i.e. not having presented an acute push of bronchopulmonary attack requiring a antibiothérapie or a hospitalization for treatment of its disease during the 2 previous weeks
- •Signed consent
- •Volunteers :
- •Men or women over 18
- •Without respiratory antecedent nor known respiratory pathology
- •Signed consent
排除标准
- •In exclusion period of an other study
- •Volunteers :
- •In exclusion period of an other study
- •Antibiotic treatment and/or anti-inflammatory drug (AINS or corticoids) in the 2 weeks before inclusion in the study
结局指标
主要结局
the DNA concentration secreted into the extracellular environment neutrophil activated
时间窗: 1 hour
次要结局
未报告次要终点