External Nitric Oxide Measurement Through SNO Degradation

Not Applicable

• Conditions: Unconscious; Healthy
• Interventions: Device: Alonefire SV003 10W 365nm UV Flashlight

• Registration Number: NCT04903535
• Lead Sponsor: Indiana University

Brief Summary

In this study, we aim to explore the feasibility of a novel, noninvasive SNO assay to acquire physiological SNO quantification from various parts of the human body and test this new method of analysis. This study aims to help with the currently cumbersome and invasive procedures used to measure SNOs in the body. The proposed activities do not unnecessarily duplicate previous experiments.

Detailed Description

S-nitrosylation is the covalent attachment of a nitric oxide group to cystine thiol within a protein to form an S-nitrosothiol (SNO); it has diverse regulatory roles in all mammalian cells and thus operates as a fundamental mechanism for cellular signaling and accounts for a large part of nitric oxide activity. Though SNOs are relevant to many biological disciplines like neuronal, muscular, respiratory, and cardiovascular biology, currently there is no way to measure SNOs that is both easy to use in a clinical setting and accurate in detecting low concentrations; detection is challenging due to the labile nature of the molecules. Low or high concentrations of these molecules could be vital indicators of incoming dangerous issues in the body. A system to easily and accurately measure SNOs could prove useful in preventative treatments. Ultra Violet (UV) light can break SNOs and can be used to measure nitric oxide release, but this method is not fully developed and further research is needed on the potential effect for UV to measure nitric oxide release.

Study Timeline

• Study First Submitted: 2021-04-29
• Study Start: 2021-05-10
• Study First Submitted QC: 2021-05-25
• Study First Posted: 2021-05-26
• Status Verified: 2024-12
• Last Update Submitted: 2024-12-05
• Last Update Posted: 2024-12-10
• Primary Completion: 2025-12-30
• Study Completion: 2025-12-30

Recruitment & Eligibility

• Status: ENROLLING_BY_INVITATION
• Sex: All
• Target Recruitment: 150

Inclusion Criteria

Not provided

Exclusion Criteria

Not provided

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
Healthy Volunteers	Alonefire SV003 10W 365nm UV Flashlight	Study will enroll to test the assay

Primary Outcome Measures

Name	Time	Method
Change in Nitric Oxide during UV light exposure	30 minutes	NO evolved during UV light exposure from the human ear lobe, hand or forehead.

Trial Locations

Locations (2)

Riley Hospital for Children; 🇺🇸 Indianapolis, Indiana, United States

Wells Center for Pediatric Research; 🇺🇸 Indianapolis, Indiana, United States