Searching for the New Mechanisms That Activate Brow Fat Tissue
Trial Snapshot
- Phase
- Not Applicable
- Status
- Completed
- Sponsor
- Enrollment
- 40
- Primary Endpoint
- The potential associations between BAT activity and dietary intake
Study Overview
Brief Summary
The general purpose of the study was to provide new information about the role of macronutrients intake, metabolomics, proteomics and microRNA on BAT activation.
The invesigator evaluated BAT activity and whole body energy consumption under cold stimulation in two gruops of healthy males aged 21-43 years old with normal BMI ( 19-25kg/m2) and in overweight/obese subjects.
Detailed Description
In detail, the main objectives of this study was to measure the influence of biological molecules of such fields: genomics, transcriptomics metabolomics and protemics and its relationship on BAT activation. The investigator also evaluated the role of diet, macronutirnets intake and polyunsaturated fatty acids on BAT activity.
Study design:
- SCREENING VISIT:
- obtaining written informed consent from all subjects
- physical examination
- body composition analysis ( DXA + bioindepedance method inbody 720)
Laboratory analyses:
Study Design
- Study Type
- Observational
- Observational Model
- Other
- Time Perspective
- Prospective
Eligibility Criteria
- Ages
- 20 Years to 43 Years (Adult)
- Sex
- Male
- Accepts Healthy Volunteers
- Yes
Inclusion Criteria
- •Inclusion criteria to the group 1 (healthy males) N=20:
- •BMI <25 kg/m2;
- •RR<140/90mmHg;
- •age 20-30/40 years
- •fasting glycemia <100mg/dl; 2h-OGTT <140mg/dl;
- •no chronic disease
- •Inclusion criteria to the group 2 ( overweight/obese males) N=20:
- •Blood pressure < 140/90 mmHg (well controlled by inh ACE; no beta-blockers)
- •No chronic disease
- •BMI > 25 <35kg/m²
- •fasting glycemia <100mg/dl; 2h-OGTT <140mg/dl;
Exclusion Criteria
- •Any drug use: beta-blokers, statins, glucocorticoids drugs, hypertension drugs;
- •hypertension;
- •hormonal disorders;
- •Inflammation process
- •Claustrophobia
Outcomes
Primary Outcomes
The potential associations between BAT activity and dietary intake
Time Frame: 2016-2018
The relationship between BAT activity and dietary intake will be analyzed
The potential associations between BAT activity and metabolite profile
Time Frame: the baseline (before cold exposure) and after 1st hour and 2nd hour of cold exposure
The potential molecules will be identified by metabolomics approach and will be analyzed dependently on the brown tissue activity level
The potential associations between BAT activity and proteins profile
Time Frame: the baseline (before cold exposure) and after 1st hour and 2nd hour of cold exposure
The potential molecules will be identified by proteomics approach and will be analyzed dependently on the brown tissue activity level
The potential associations between BAT activity and transcriptome profile
Time Frame: the baseline (before cold exposure) and after 1st hour and 2nd hour of cold exposure
The potential molecules will be identified by transriptomics approach and will be analyzed dependently on the brown tissue activity level
Secondary Outcomes
- The potential associations between BAT activity and irisin concentrations(the baseline (before cold exposure) and after 1st hour and 2nd hour of cold exposure)
- The potential associations between BAT activity and atrial natiuretic peptide concentrations(the baseline (before cold exposure) and after 1st hour and 2nd hour of cold exposure)
- The potential associations between BAT activity and fT3 concentrations(the baseline (before cold exposure) and after 1st hour and 2nd hour of cold exposure)
- The potential associations between BAT activity and Interleukin-6 concentrations(the baseline (before cold exposure) and after 1st hour and 2nd hour of cold exposure)
- The potential associations between BAT activity and FFA ( free fatty acids) concentrations(the baseline (before cold exposure) and after 1st hour and 2nd hour of cold exposure)
- The potential associations between BAT activity and energy expenditure(at the baseline ( -30 min to 0 min) and every 30 min until 120 min of cold exposure)
- The potential associations between BAT activity and glucose concentrations(he baseline (before cold exposure) and after 1st hour and 2nd hour of cold exposure)
- The potential associations between BAT activity and TSH concentrations(the baseline (before cold exposure) and after 1st hour and 2nd hour of cold exposure)
- The potential associations between BAT activity and substrates utilization(at the baseline ( -30 min to 0 min) and every 30 min until 120 min of cold exposure.)
- The potential associations between BAT activity and insulin concentrations(the baseline (before cold exposure) and after 1st hour and 2nd hour of cold exposure)
- The potential associations between BAT activity and brain natiuretic peptide concentrations(the baseline (before cold exposure) and after 1st hour and 2nd hour of cold exposure)
- The potential associations between BAT activity and plasma nonesterified fatty acid concentrations(the baseline (before cold exposure) and after 1st hour and 2nd hour of cold exposure)
- The potential associations between BAT activity and fT4 concentrations(the baseline (before cold exposure) and after 1st hour and 2nd hour of cold exposu)