Control of Leukocyte Function

Completed

• Conditions: Inflammation, Brain

• Registration Number: NCT03027232
• Lead Sponsor: Beth Israel Deaconess Medical Center

Brief Summary

The protocol is to draw peripheral blood from healthy volunteers for in vitro studies. The aims of these in vitro studies are to determine the cellular and intracellular mechanisms by which hypertonic saline and ATP release regulate neutrophil and lymphocyte functions.

Detailed Description

The proper regulation of leukocyte function is critical for immune defense and to prevent autoimmune diseases. Many aspects of immune cell regulation are unknown. A better understanding could lead to novel therapeutic approaches to improve immune function in patients suffering from various diseases.

The investigators have found that hypertonic conditions regulate leukocyte functions through feedback mechanisms involving ATP release and purinergic receptors. The purpose of the proposed protocol is to obtain access to peripheral blood from healthy volunteers to study these complex regulatory mechanisms in more detail.

Study Timeline

• Study Start: 2007-01
• Study First Submitted: 2017-01-13
• Study First Submitted QC: 2017-01-20
• Study First Posted: 2017-01-23
• Primary Completion: 2017-12-15
• Study Completion: 2017-12-15
• Status Verified: 2018-04
• Last Update Submitted: 2018-04-26
• Last Update Posted: 2018-04-27

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 100

Inclusion Criteria

• any healthy volunteer

Exclusion Criteria

• pregnancy
• needing/using drugs
• inflammatory or infectious complications

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Immune cells will be studied in vitro to learn about basic mechanisms of immune cell signaling.	Blood is collected as needed; time frame: indefinitely. Blood used between 1-7 days after blood draws.	The purpose of this study is to obtain blood from healthy subjects (volunteers) for in vitro studies to explore how immune cells function. Cells will be isolated and subjected to various tests including stimuli that simulate immune cell activation in health and disease. Subjects will not be subjected to any treatments. Only some of the cells (leukocytes) in the blood will be studied. For example, activation events such as cytokine production, the ability of leukocytes (=immune cells) to detect and respond to bacterial products will be assessed. No identifiable information about study subjects will be kept.

Trial Locations

Locations (1)

BIDMC; 🇺🇸 Boston, Massachusetts, United States