Potential Biomarkers of the Severity of Endothelial Damage in End-stage Chronic Kidney Failure Evaluated at the Initiation of Intermittent or Peritoneal Dialysis
试验速览
- 阶段
- 不适用
- 状态
- 终止
- 入组人数
- 10
- 试验地点
- 2
- 主要终点
- Activation of 5 proteins relocated in the membrane of monocytes (eNOS, iNOS, gp91phox and receptors of angiotensin II (AT1 and AT2)) within dynamic lipid structures, rafts, measured by western blot-dot blot.
研究概览
简要总结
The principal objective of this study is to qualify markers of oxidative stress in inflammatory cells (monocytes) in patients with stage 3 kidney failure (diabetic or not), and patients with end-stage kidney failure (diabetic or not), who require dialysis. The evaluation of these markers will be done by the activation and localization of proteins implicated in vascular tone and oxidative stress in monocytes, correlated with the distribution of cholesterol sphingomyelin within planar rafts and caveolae. The aim is to describe their evolution under treatment, which could lead to interventional studies.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Parallel
- 主要目的
- Other
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 75 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Patient who have been informed about the research
- •Patients with national health insurance cover
- •Patients aged between 18 and 75 years
- •Patients with stage 3 kidney failure (30 ml/min < cl creat < 59 ml/min) diabetic or not
- •Patients with stage 5 kidney failure (cl creat < 15 ml/mn) diabetic or not and requiring dialysis.
排除标准
- •Patients under guardianship
- •Pregnant or breast-feeding women
- •Infection (including peritonitis in peritoneal dialysis, infection of the catheter insertion site),
- •Neoplastic disease,
- •Systemic diseases in flare,
- •Patients positive for Human Immunodeficience Virus (HIV),
- •Patients on immunosuppressants
- •Patients taking antioxidants (selenium, vitamin C and/or E)
- •Patient on statins
研究组 & 干预措施
group with stage 3 kidney failure (diabetic or not)
干预措施: Blood sample (Biological)
group with stage 3 kidney failure (diabetic or not)
干预措施: Retinal photography: (Other)
group with stage 5 kidney failure (diabetic or not)
干预措施: Blood sample (Biological)
group with stage 5 kidney failure (diabetic or not)
干预措施: dialyse (Other)
结局指标
主要结局
Activation of 5 proteins relocated in the membrane of monocytes (eNOS, iNOS, gp91phox and receptors of angiotensin II (AT1 and AT2)) within dynamic lipid structures, rafts, measured by western blot-dot blot.
时间窗: at the beginning of the period Day 0, at 1 month and at 3 months.
次要结局
未报告次要终点