Immune Benefits of Consumption of Dairy Yogurt Containing Functional Probiotics Among Individuals Over 60 Years Old
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 200
- 试验地点
- 2
- 主要终点
- Natural killer cell activity
研究概览
简要总结
To investigate the impact of consuming dairy yogurt on immun function including natural killer (NK) cell activity, circulating levels of cytokines, and immunoglobulin (Ig) in elderly.
详细描述
A randomized, open-labelled, placebo-controlled study was conducted on 200 nondiabetic, nonobese and elderly (≥60 years) subjects. Over a twelve-week period, the test group consumed one bottle (120 mL) of dairy yogurt each day, whereas the placebo group consumed same volume of low fat milk daily. NK cell activity (%) was measured based on the ratios of effector cells (E; peripheral blood mononuclear cells, PBMCs) from each participant to target cells (T; K562 cells) at E:T= 10:1, 5:1, 2.5:1, 1.25:1 or 0.625:1. In addition, cytokines and immunoglobulin levels measured using commercial kits as manufacturer's instruction.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Supportive Care
- 盲法
- None
入排标准
- 年龄范围
- 60 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Able to give informed consent
- •Over 60 years old without any health problems
- •The levels of white blood cells within range 4x10^3/μL~10x10^3/μL
排除标准
- •Individuals who get a cold
- •Hypersensitivity or disease history for milk protein
- •Epilepsy, neurological, or psychological disease
- •Alcoholism or drug addiction
- •Liver disease or severe kidney failure disease
- •Inflammation-related disease
- •Consumption of other test products or drugs within 1 month prior to screening
- •Regular consumption of immune-related functional foods including yogurt or milk within 1 month prior to screening
- •History of inflammation-related disease or taking medication to treat inflammation-related disease within 1 month prior to screening
- •History of cancer within 5 years
- •Any inappropriate condition
结局指标
主要结局
Natural killer cell activity
时间窗: At 12-week follow-up
Change from baseline natural killer cell activity at 12-week
时间窗: At baseline and 12-week follow-up
次要结局
- Interleukin-12(At 12-week follow-up)
- Change from baseline immunoglobulin at 12-week(At baseline and 12-week follow-up)
- Interferon-gamma(At 12-week follow-up)
- White blood cell(At 12-week follow-up)
- Change from baseline Interferon-gamma at 12-week(At baseline and 12-week follow-up)
- Change from baseline white blood cell at 12-week(At baseline and 12-week follow-up)
- Change from baseline Interleukin-12 at 12-week(At baseline and 12-week follow-up)
- Immunoglobulin(At 12-week follow-up)