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Visualize Nociceptor Changes in Neuropathic Human

Not Applicable
Conditions
Chemotherapy-induced Peripheral Neuropathy
Peripheral Neuropathy
Interventions
Diagnostic Test: harmonic generation (HGM) microscopic methods.
Registration Number
NCT03112057
Lead Sponsor
National Taiwan University Hospital
Brief Summary

The purpose of this experiment is to establish a solid medical foundation for the successful development of non-invasive harmonic generation (HGM) microscopic slicing methods. The purpose of this new technology platform is to directly observe the nerve endings of nociceptors To enhance understanding of how neuropathies occur, persistence, and ease. This technique will assist in the diagnosis and prognosis of patients with neuralgia.

Detailed Description

This study will be the skin tissue for the virtual slice object, the study intends to the following research:

The number of people enrolled in the Court is 170.

Between 20 and 90 years old, meet the following conditions, suitable for participation in this test:

1. Healthy subjects of different ages (20 persons): to study the optical imaging of the skin and nerve endings: the control of healthy subjects and peripheral neuropathy patients skin observed by the optical images of the shoots.

2. Abandoned extra digit specimen of polydactyly(10 persons): to study the optical imaging of the normal skin and nerve endings in extra digit : collect abandoned digit specimen after surgical removal in polydactyly.

3. has been diagnosed with peripheral neuropathy (90 persons):to study the diagnosis of peripheral neuropathy of the skin nerve endings of the optical image observation and quantitative sensory nerve examination

4. clinically must be diagnosed by the skin slices of diabetic neuropathy patients have been removed after the completion of clinical diagnosis of the specimen,And then the skin slices of the nerve endings of the optical image observation (20 persons):study the technical observation of diabetic patients with skin sections of the nerve endings of the optical image observation: the clinical must be skin slices for the diagnosis of diabetic neuropathy, patients has been taken out after the completion of clinical diagnosis, and then the skin slices of the nerve endings of the light learning image observation.

5. because of chemotherapy during chemotherapy, is expected to have peripheral neuropathy: before the occurrence of peripheral neuropathy , Peripheral nerve lesions were cured after subcutaneous nerve optical observation (30 persons):for different periods of peripheral neuropathy subcutaneous nerve optical observation: in the peripheral neuropathy occurred before the occurrence of peripheral neuropathic symptoms, peripheral neuropathy after cure for subcutaneous nerve optical observation.

This test can not be attended if:

Those who suffer from systemic infection or have been infected with the site by the physician assessment is not suitable for examination.

Recruitment & Eligibility

Status
UNKNOWN
Sex
All
Target Recruitment
170
Inclusion Criteria
  • Healthy subjects of different ages
  • abandoned extra digit specimen in polydactyly
  • diagnosed with peripheral neuropathy
  • diabetic neuropathy patients have been removed after the completion of clinical diagnosis of the specimen, And then the skin slices of the nerve endings of the optical image observation
  • because of chemotherapy during chemotherapy, is expected to have peripheral neuropathy: before the occurrence of peripheral neuropathy , after cured peripheral nerve lesions
Exclusion Criteria
  • systemic infection or have been infected with the site by the physician assessment is not suitable for examination.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
peripheral neuropathyharmonic generation (HGM) microscopic methods.the participant have symptoms and diagnosed with peripheral neuropathy (90 persons), Interventions:harmonic generation microscopy
diabetic neuropathyharmonic generation (HGM) microscopic methods.the participant have symptoms and diagnosed with diabetic neuropathy (20 persons), Interventions:harmonic generation microscopy
chemotherapy induced neuropathyharmonic generation (HGM) microscopic methods.before chemotherapy, during chemotherapy, after peripheral nerve lesions were cured (30 persons). Interventions:harmonic generation microscopy
Healthy subjectsharmonic generation (HGM) microscopic methods.Healthy subjects of different ages (20 persons), Interventions: harmonic generation microscopy
Polydactylyharmonic generation (HGM) microscopic methods.Abandoned extra digit specimen of polydactyly(10 persons): the normal skin and nerve endings in extra digit: Interventions: harmonic generation microscopy
Primary Outcome Measures
NameTimeMethod
nerve ending number measurementthrough study completion, an average of 1 year

Use of harmonic generation (HGM) microscopic virtual biopsy methods to obtain 3D live images.

Secondary Outcome Measures
NameTimeMethod
nerve ending structure measurementthrough study completion, an average of 1 year

Quantify the molecules on the image, the biological characteristics of tissue nerve cells have a comprehensive understanding, and will try to establish the human subcutaneous nerve optical observation method

Trial Locations

Locations (1)

National Taiwan University Hospital

🇨🇳

Taipei, Taiwan

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