Clinical and Microbiological Evaluation of Probiotics as an Adjunct to Initial Periodontal Treatment in Chronic Periodontitis
Trial Snapshot
- Phase
- Not Applicable
- Status
- Completed
- Sponsor
- Marmara University
- Enrollment
- 30
- Locations
- 2
- Primary Endpoint
- Probing Depth
Study Overview
Brief Summary
The aim of this study is to evaluate the clinical and microbiological effects of Bifidobacterium animalis subsp. lactis DN-173010 containing yoghurt as an adjunct to mechanical periodontal treatment.
Detailed Description
The aim of this study is to evaluate the clinical and microbiological effects of Bifidobacterium animalis subsp. lactis DN-173010 containing yoghurt as an adjunct to mechanical periodontal treatment.
Thirty chronic periodontitis patients were randomized into 2 groups. As adjunctive to MPT, Group 1 consumed 2×108 CFU/day of Bifidobacterium animalis subsp. lactis DN-173010 containing probiotic yoghurt while Group 2 natural yoghurt once daily for 28 days. Subgingival plaque samples were obtained from 2 periodontal sites with probing pocket depth (PPD) of 4≤PPD<7 mm via paper-points at baseline, 28th day, 3rd month. Samples were cultured to evaluate total viable count (TVC), the proportions of obligate anaerobes (OA) and Bifidobacterium species (B) (TVC%). The full-mouth recorded clinical parameters were plaque index, gingival index, PPD, bleeding on probing, and clinical attachment level.
Study Design
- Study Type
- Interventional
- Allocation
- Randomized
- Intervention Model
- Parallel
- Primary Purpose
- Treatment
- Masking
- Double (Participant, Care Provider)
Masking Description
Individuals and doctor were blinded to the study groups allocation. Before statistical analysis, the blind study coordinator broke the code to allocate the indivuals to the groups.
Eligibility Criteria
- Ages
- 18 Years to — (Adult, Older Adult)
- Sex
- All
- Accepts Healthy Volunteers
- Yes
Inclusion Criteria
- •chronic periodontitis patients with radiographically detected horizontal bone loss
- •the presence of at least 2 teeth with one approximal site with a probing pocket depth (PPD) of 4≤SD<7 mm (5-7 mm) and a GI of ≥2 mm in each quadrant,
- •previously untreated periodontitis, systemically healthy
Exclusion Criteria
- •Systemic diseases
- •Antibiotics and anti-inflammatory drugs within 3 months before the study
- •Untreated caries lesions or local retention factors
- •Allergic reactions to lactose or fermented milk products
- •Previous probiotic supplements in diet
- •Pregnancy
Outcomes
Primary Outcomes
Probing Depth
Time Frame: 28 days
Probing depth is defined as the distance from the free gingival margin to the bottom of the periodontal pocket.
Secondary Outcomes
- Gingival Index(28 days)
- Plaque Index(28 days)
- Clinical attachment level(28 days)
- microbiological parameters(28 days)
- Bleeding on probing(28 days)