Action of teeth treatment on fungal growth in mouth of HIV infected children under HIV treatment.

Completed

Conditions: Human immunodeficiency virus [HIV]disease,

Registration Number: CTRI/2020/03/024363

Lead Sponsor: Monica Gurupriya M

Brief Summary: Thirty children from the Home for needs (Tiruvallur) who fit with inclusion criteria and are willing to participate, shall be enrolled. The children will be introduced and subjected to OHIP-14 questionnaire to assess their oral health related quality of life(OHRQoL) and baseline oral swab will be collected to assess the Candida albicans carriage in the oral cavity. Swab sample will be taken by rolling the sterile cotton swab across the dorsum of the tongue, vestibular sulcus and peak of the palatal vault. Swab sample will be immediately smeared onto the surface of culture plate with Sabouraudâ€™s dextrose agar(100gm)(with chloramphenicol 10%).The collected swabs will be transported in cold chain maintained at 4 degree C within 2hours from the time of collection. Plates will be aerobically incubated at 37Â° centigrade for 48 to 72 hours. Number of *Candida* colonies will be determined by counting Colony Forming Units on Sabouraudâ€™s dextrose agar plates. Preliminary identification of *Candida* will be carried out after the growth of characteristic creamy convex yeast colonies on SDA. All the microbiological analysis will be done at Standard Healthcare Multi-Speciality clinic and Diagnostic Centre at Pallikaranai, Chennai. This will be followed by dental treatment such as restoration and extraction. Oral hygiene kit will be provided commonly to all the participants to maintain homogeneity in oral care. Daily reminders about oral hygiene will be given through phone calls to all participants. Four weeks after finishing the dental treatment, another oral swab sample will be collected from the patients according to the criteria described previously. Post-treatment OHIP-14 questionnaire will be administered finally.

Detailed Description: Not available

Recruitment & Eligibility

Status: Completed

Sex: All

Target Recruitment: 30

Inclusion Criteria

Children with well-controlled HIV infection (Under HAART with CD4 count >200cell/mm 3) for the past 1 year.
Children who agreed to participate, and whose parents / guardian give consent.
Consent was also obtained from the Head of the Institution.
3.Children with 1 or more carious teeth.

Exclusion Criteria

1.Children who gave history of prolonged antibiotic treatment.
2.Children with fungal infection in the mouth / under antifungal therapy.
3.Children with chronic diseases, such as diabetes and allergies.
4.Children with any developmental anomalies of teeth.
5..Patients who were psychologically compromised(mental retardation , etc.).

Study & Design

Study Type: Observational

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Post dental treatment-Oral health related quality of life.	Baseline,4weeks after dental treatment.

Secondary Outcome Measures

Name	Time	Method
Post dental treatment Candida albicans count.	4months(April-july)

Trial Locations

Locations (1): Home for needs,Thiruvallur
🇮🇳
Thiruvallur, TAMIL NADU, India
Home for needs,Thiruvallur
🇮🇳Thiruvallur, TAMIL NADU, India
Mr Joseph
Principal investigator
9944478745
joseph_s@wvi.org