Ovarian Platelet-Rich Plasma (oPRP) Injections Improvement In IVF Patients Based On Time-Lapse Incubator Culture for Automated Tracking and AI for Embryo Quality Assessment : A Non-Randomized Propsective Self-Controlled Interventional Study

. oPRP Treatment & Second IVF Cycle (Study Cycle)

• Following the first IVF cycle, patients will undergo their first oPRP injection:

• Timing: 1-2 weeks after the onset of the next menstrual cycle.

• Procedure: Under IV sedation, a patient's autologous PRP will be prepared and injected directly into both ovaries under transvaginal ultrasound guidance.

• Second IVF Cycle (oPRP Study Cycle)

• The same ovarian stimulation protocol will be used for consistency.

• A second oPRP injection will be performed on stimulation days 2-5 to enhance folliculogenesis.

• Ovulation will be triggered at the same follicular size thresholds, and oocyte retrieval will follow identical protocols.

• Fertilization methodology will be identical to the first cycle unless no fertilization is identified in the first cycle.

• Embryo Development Analysis: Up to 15 embryos will be cultured in the AI incubator, where time-lapse imaging will track their morphokinetics, and AI-based analysis will assess embryo viability and implantation potential.