Nasal Epithelium Gene Expression Profiling in Child Respiratory Allergic Disease

Not Applicable

Completed

• Conditions: Allergic Rhinitis
• Interventions: Procedure: Collection of nasal epithelial cells

• Registration Number: NCT00569361
• Lead Sponsor: Centre Hospitalier Universitaire de Nice

Brief Summary

Using a human pangenomic microarray, the researchers established expression profiles of nasal epithelial cells, collected by brushing of patients belonging to one of four distinct groups:

1. allergic rhinitis to dust mite (AR) isolated (n=12),

2. AR associated with bronchial hyperreactivity (n=12),

3. AR associated with asthma (n=14),

4. control (n=14).

Detailed Description

Context: Asthma is the most frequent chronic disease in childhood. Allergic rhinitis has been described as a risk factor to develop asthma. The objective of the study was to evaluate the contribution of the respiratory epithelium to development of allergic rhinitis and asthma and to identify the molecular mechanisms driving rhinitis toward asthma.

Methods: Using a human pangenomic microarray, we established expression profiles of nasal epithelial cells, collected by brushing of patients belonging to one of four distinct children 2 to 18 years of age groups:

1. allergic rhinitis to dust mite (AR) isolated (n=12),

2. AR associated with bronchial hyperreactivity (n=12),

3. AR associated with asthma (n=14),

4. control (n=14).

Study Timeline

• Study Start: 2004-11
• Study Completion: 2005-10
• Study First Submitted: 2007-12-06
• Study First Submitted QC: 2007-12-06
• Study First Posted: 2007-12-07
• Status Verified: 2009-06
• Last Update Submitted: 2009-06-08
• Last Update Posted: 2009-06-09

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 80

Inclusion Criteria

Patients:

• Patients between 6 and 17 years old
• Patients with allergic rhinitis

Witness:

• Patients between 6 and 17 years old
• Patients without allergic rhinitis and asthma

Exclusion Criteria

• Rhino-bronchitis infection dated from less than 15 days
• Patients younger than 6 or older than 17 years

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
A	Collection of nasal epithelial cells	Collection of nasal epithelial cells by brushing

Primary Outcome Measures

Name	Time	Method
Transcriptome analysis: genes statistically modulated between groups, predictive biomarkers allowing correct classification of groups	At the sample time

Secondary Outcome Measures

Name	Time	Method
Validation with other techniques: Q-PCR	At the sample time

Trial Locations

Locations (1)

Department of Pediatrics, Nice University Hospital; 🇫🇷 Nice, France