Nasal Epithelium Gene Expression Profiling in Child Respiratory Allergic
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 80
- 试验地点
- 2
- 主要终点
- Transcriptome analysis: genes statistically modulated between groups, predictive biomarkers allowing correct classification of groups
研究概览
简要总结
Using a human pangenomic microarray, the researchers established expression profiles of nasal epithelial cells, collected by brushing of patients belonging to one of four distinct groups:
- allergic rhinitis to dust mite (AR) isolated (n=12),
- AR associated with bronchial hyperreactivity (n=12),
- AR associated with asthma (n=14),
- control (n=14).
详细描述
Context: Asthma is the most frequent chronic disease in childhood. Allergic rhinitis has been described as a risk factor to develop asthma. The objective of the study was to evaluate the contribution of the respiratory epithelium to development of allergic rhinitis and asthma and to identify the molecular mechanisms driving rhinitis toward asthma.
Methods: Using a human pangenomic microarray, we established expression profiles of nasal epithelial cells, collected by brushing of patients belonging to one of four distinct children 2 to 18 years of age groups:
- allergic rhinitis to dust mite (AR) isolated (n=12),
- AR associated with bronchial hyperreactivity (n=12),
- AR associated with asthma (n=14),
- control (n=14).
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Screening
- 盲法
- None
入排标准
- 年龄范围
- 6 Years 至 17 Years（Child）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Patients between 6 and 17 years old
- •Patients with allergic rhinitis
- •Patients between 6 and 17 years old
- •Patients without allergic rhinitis and asthma
排除标准
- •Rhino-bronchitis infection dated from less than 15 days
- •Patients younger than 6 or older than 17 years
结局指标
主要结局
Transcriptome analysis: genes statistically modulated between groups, predictive biomarkers allowing correct classification of groups
时间窗: At the sample time
次要结局
- Validation with other techniques: Q-PCR(At the sample time)