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Thiotepa, Busulfan and Fludarabin for pt With Refractory/Early Relapsed Aggressive B-cell Non Hodgkin Lymphomas

Phase 2
Conditions
B-cell Lymphoma Refractory
Interventions
Drug: Fludarabin
Procedure: transplant (HCT)
Radiation: Cytoreduction
Drug: Immunosuppression
Procedure: Collection and infusions of Donor PBSC
Registration Number
NCT01786018
Lead Sponsor
Azienda Ospedaliera San Giovanni Battista
Brief Summary

The purpose of this study is to evaluate progression free survival, transplant-related morbidity (TRM) at day +100 and at +365, overall survival and incidence of acute and chronic GVHD in refractory/early relapsed aggressive B-cell non Hodgkin lymphomas patients treated with allogeneic Transplantation after a conditioning with Thiotepa, Busulfan and fludarabin.

Detailed Description

In the present study, it is hypothesised that patients with aggressive B cell lymphomas refractory to or relapsed early (within 12 months) after the completion of standard first-line immunoProtocol TBF2012 Version 1, 20 Nov 2012 9 chemotherapy can benefit from de-bulking salvage therapy (i.e. R-DHAP + bortezomib) followed by an allograft to improve progression-free survival.

Patient inclusion criteria

* Patients with refractory/relapsed aggressive B-cell non Hodgkin lymphomas after frontline therapy.

* Patients with stable disease or partial or complete remission (PET-negative) after salvage therapy

* Patients younger than 65 years old

* A fully HLA-identical sibling or matched unrelated donor is available. Patients with one antigen mismatched donors can be considered

* Patient must be competent to give consent

Patient exclusion criteria

* Patients treated with an autologous transplant as salvage therapy

* Patients with progressive lymphomas despite conventional therapies

* Patients with progressive lymphomas despite conventional therapies

* Uncontrolled CNS involvement with disease

* Fertile men or women unwilling to use contraceptive techniques during and for 12 months following treatment

* Females who are pregnant or breastfeeding

* Organ dysfunction defined as follows:

* Cardiac function: ejection fraction \<30% or uncontrolled cardiac failure

* Pulmonary: DLCO \<40% predicted

* Liver function abnormalities: elevation of bilirubin to \> 3 mg/dl and/or transaminases \>4 the upper limit of normal

* Renal: creatinine clearance \<50 cc/min (24 hour urine Protocol TBF2012 Version 1, 20 Nov 2012 6 collection)

* Karnofsky performance score \< 60%

* Patients with poorly controlled hypertension despite multiple antihypertensives

* Documented fungal disease that is progressive despite treatment

* Viral infections: HIV positive patients.

* Positive serology for Hepatitis B (HB) defined as a positive test for HBsAg. In addition, if negative for HBsAg but HBcAb positive (regardless of HBsAb status), a HBV DNA test will be performed and if positive the subject will be excluded.

* Positive serology for hepatitis C (HC) defined as a positive test for HCAb, in which case reflexively perform a HC RIBA immunoblot assay on the same sample to confirm the result

* Psychiatric disorders or psychosocial problems which in the opinion of the primary physician or Principal Investigator would place the patient at unacceptable risk from this regimen.

* Patients with active non-hematologic malignancies (except nonmelanoma skin cancers).

* Patients with a history of non-hematologic malignancies (except non-melanoma skin cancers) currently in a complete remission, who are less than 5 years from the time of complete remission, and have a \>20% risk of disease recurrence. Donor inclusion criteria:

* Related or unrelated HLA identical donors who are in good health and have no contra-indication to donation. One antigen HLAmismatched (9/10 match) donors will also be considered.

* No contra-indication for the donor to collection by apheresis of mononuclear cells mobilized by G-CSF at a dose of 10-12 μg/kg of body weight.

* Donor must have adequate veins for leukapheresis or agree to placement of central venous catheter (femoral, subclavian). Donor exclusion criteria:

* Age \< 18 years.

* Identical twin.

* Pregnancy.

* Infection with HIV.

* Inability to achieve adequate venous access.

* Known allergy to filgrastin (G-CSF).

* Current serious systemic illness.

Donor inclusion criteria:

* Related or unrelated HLA identical donors who are in good health and have no contra-indication to donation. One antigen HLAmismatched (9/10 match) donors will also be considered.

* No contra-indication for the donor to collection by apheresis of mononuclear cells mobilized by G-CSF at a dose of 10-12 μg/kg of body weight.

* Donor must have adequate veins for leukapheresis or agree to placement of central venous catheter (femoral, subclavian). Donor exclusion criteria:

* Age \< 18 years.

* Identical twin.

* Pregnancy.

* Infection with HIV.

* Inability to achieve adequate venous access.

* Known allergy to filgrastin (G-CSF).

* Current serious systemic illness.

Donor inclusion criteria:

* Related or unrelated HLA identical donors who are in good health and have no contra-indication to donation. One antigen HLAmismatched (9/10 match) donors will also be considered.

* No contra-indication for the donor to collection by apheresis of mononuclear cells mobilized by G-CSF at a dose of 10-12 μg/kg of body weight.

* Donor must have adequate veins for leukapheresis or agree to placement of central venous catheter (femoral, subclavian). Donor exclusion criteria:

* Age \< 18 years.

* Identical twin.

* Pregnancy.

* Infection with HIV.

* Inability to achieve adequate venous access.

* Known allergy to filgrastin (G-CSF).

* Current serious systemic illness.

Recruitment & Eligibility

Status
UNKNOWN
Sex
All
Target Recruitment
42
Inclusion Criteria

Not provided

Exclusion Criteria

Not provided

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
1Thiotepa* Thiotepa 5 mg/kg/day on days -7, -6 (Total Dose 10 mg/kg) * Busulfan (i.v.) 3.2 mg/kg on days -5,-4,-3 (Total Dose 9.6 mg/kg) * Fludarabin: 50 mg/m2/day on days -5,-4,-3 (Total Dose 150 mg/m2)
1transplant (HCT)* Thiotepa 5 mg/kg/day on days -7, -6 (Total Dose 10 mg/kg) * Busulfan (i.v.) 3.2 mg/kg on days -5,-4,-3 (Total Dose 9.6 mg/kg) * Fludarabin: 50 mg/m2/day on days -5,-4,-3 (Total Dose 150 mg/m2)
1Fludarabin* Thiotepa 5 mg/kg/day on days -7, -6 (Total Dose 10 mg/kg) * Busulfan (i.v.) 3.2 mg/kg on days -5,-4,-3 (Total Dose 9.6 mg/kg) * Fludarabin: 50 mg/m2/day on days -5,-4,-3 (Total Dose 150 mg/m2)
1Cytoreduction* Thiotepa 5 mg/kg/day on days -7, -6 (Total Dose 10 mg/kg) * Busulfan (i.v.) 3.2 mg/kg on days -5,-4,-3 (Total Dose 9.6 mg/kg) * Fludarabin: 50 mg/m2/day on days -5,-4,-3 (Total Dose 150 mg/m2)
1Collection and infusions of Donor PBSC* Thiotepa 5 mg/kg/day on days -7, -6 (Total Dose 10 mg/kg) * Busulfan (i.v.) 3.2 mg/kg on days -5,-4,-3 (Total Dose 9.6 mg/kg) * Fludarabin: 50 mg/m2/day on days -5,-4,-3 (Total Dose 150 mg/m2)
1Immunosuppression* Thiotepa 5 mg/kg/day on days -7, -6 (Total Dose 10 mg/kg) * Busulfan (i.v.) 3.2 mg/kg on days -5,-4,-3 (Total Dose 9.6 mg/kg) * Fludarabin: 50 mg/m2/day on days -5,-4,-3 (Total Dose 150 mg/m2)
1Busulfan* Thiotepa 5 mg/kg/day on days -7, -6 (Total Dose 10 mg/kg) * Busulfan (i.v.) 3.2 mg/kg on days -5,-4,-3 (Total Dose 9.6 mg/kg) * Fludarabin: 50 mg/m2/day on days -5,-4,-3 (Total Dose 150 mg/m2)
1Methotrexate* Thiotepa 5 mg/kg/day on days -7, -6 (Total Dose 10 mg/kg) * Busulfan (i.v.) 3.2 mg/kg on days -5,-4,-3 (Total Dose 9.6 mg/kg) * Fludarabin: 50 mg/m2/day on days -5,-4,-3 (Total Dose 150 mg/m2)
1Cyclosporine* Thiotepa 5 mg/kg/day on days -7, -6 (Total Dose 10 mg/kg) * Busulfan (i.v.) 3.2 mg/kg on days -5,-4,-3 (Total Dose 9.6 mg/kg) * Fludarabin: 50 mg/m2/day on days -5,-4,-3 (Total Dose 150 mg/m2)
1ATG* Thiotepa 5 mg/kg/day on days -7, -6 (Total Dose 10 mg/kg) * Busulfan (i.v.) 3.2 mg/kg on days -5,-4,-3 (Total Dose 9.6 mg/kg) * Fludarabin: 50 mg/m2/day on days -5,-4,-3 (Total Dose 150 mg/m2)
Primary Outcome Measures
NameTimeMethod
Progression free survival2 years
Secondary Outcome Measures
NameTimeMethod
Incidence of acute and chronic GVHD2 years
Transplant-related morbidity (TRM) at day +100 and at +3651 year
Overall survival2 years

Trial Locations

Locations (1)

Città della Salute e della Scienza di Torino

🇮🇹

Torino, Italy

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