Adding Insulin to IVF Culture Media Significantly Improve Outcome
Trial Snapshot
- Phase
- Not Applicable
- Status
- Completed
- Sponsor
- Ibn Sina Hospital
- Enrollment
- 360
- Locations
- 2
- Primary Endpoint
- clinical Pregnancy rate ( % )
Study Overview
Brief Summary
Add insulin to a single step culture media for IVF embryo culture from day 0 to day 5/6 of In Vitro culture and detect the the effect upon embryo quality and implantation potential.
Detailed Description
The IVF culture media design and upgrade underwent many ideas during the last 3 decades, we suppose that adding insulin as a hormon and growth factor might improve embryo development in vitro so we decide to design a trial to compare the embryogenesis by sibling Oocytes of the same patient between to arm of the same culture media and yhe same culture condition but with one arm with insulin and the other is traditional one and closely monitor the outcome within the 2 arms abd record the results
Study Design
- Study Type
- Interventional
- Allocation
- Randomized
- Intervention Model
- Parallel
- Primary Purpose
- Basic Science
- Masking
- None
Eligibility Criteria
- Ages
- 18 Years to 40 Years (Adult)
- Sex
- Female
- Accepts Healthy Volunteers
- Yes
Inclusion Criteria
- •normal responder
- •Tubal factor infertility
- •mild asthenozoospermia
Exclusion Criteria
- •patient over 40
Arms & Interventions
In Vitro culture media with insulin
We will add insulin to the single step culture media to monitor the embryogenesis
Intervention: Adding insulin to In Vitro culture media (Other)
In Vitro culture media without insulin m
The embryos will be cultured in the media without insulin and compare this arm as the control arm with the interventional one.
Outcomes
Primary Outcomes
clinical Pregnancy rate ( % )
Time Frame: 6 months
Secondary Outcomes
- Blastocyst rate ( % )(6 months)
- Blastocyst quality rate ( % )(6 months)
Investigators
Muhammad Fawzy
IVF Laboratory Director
Ibn Sina Hospital