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Periodontal Regeneration With Recombinant Human Platelet Derived Growth Factor (rH-PDGF-BB) and Allograft

Phase 2
Recruiting
Conditions
Periodontal Diseases
Interventions
Combination Product: Allograft and rHPDGF-BB
Combination Product: Allograft and Saline solution
Registration Number
NCT06162832
Lead Sponsor
Universidad Autonoma de Nuevo Leon
Brief Summary

Growth factors are defined as small proteins that trigger a cellular response after binding to cell receptors; Tissue engineering is now clinically applicable in a commercially available system involving the use of recombinant human platelet-derived growth factor.

The objective of this study is to evaluate clinical and radiographic parameters in bone defects treated with platelet-derived growth factor in combination with allograft.

Our hypothesis: Defects treated with DFDBA and rhPDGF-BB have better clinically and radiographic results that the defects treated with DFDBA and saline solution.

Detailed Description

The study will be performed in 30 periodontal defects of 2 and 3 walls. The experimental group will consist of DFDBA placement with rhPDGF-BB and control DFDBA with saline solution. The clinical parameters recorded were Probing deep (PD), clinical attachment level (CAL), recession depth, Index of bleeding and plaque at baseline and at six months. Our universe is patients with periodontitis attending at the clinical in Periodontics at the Autonomous University of Nuevo Leon (UANL) School of Dentistry.

Recruitment & Eligibility

Status
RECRUITING
Sex
All
Target Recruitment
30
Inclusion Criteria
  • Clinical diagnosis of periodontitis
  • Infrabony defects of 2 walls, 3 walls and combined defects
  • Patients ASA (American Society of Anesthesiologists) I and II
  • Patients who smoke less than 10 cigarettes
Exclusion Criteria
  • Patients undergoing bisphosphonate therapy
  • Furcation defects
  • Patients with evidence of blood dyscrasias.
  • Pregnant patient

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
AllograftAllograft and Saline solutionWill be used Demineralized cancellous allograft 250-1000um
AllograftAllograft and rHPDGF-BBWill be used Demineralized cancellous allograft 250-1000um
Saline SolutionAllograft and Saline solutionThe saline solution will be used for hydrate the bone graft 20 minutes before the placement at the defect
rhPDGF-BB (GEM21, Lynch Biologics)Allograft and rHPDGF-BBThe rhPDGF-BB will be used for hydrate the bone graft 20 minutes before the placement at the defect
Primary Outcome Measures
NameTimeMethod
Gingival RecessionInitial and six months after surgery

The distance from the soft tissue (gingiva or alveolar mucosa) margin to cemento-enamel junction, ≥1 millimeters (mm)

Radiographic MeasurementsInitial and six months after surgery

Cemento-enamel junction (CEJ)-Defect Base (DB): The distance of the cementoenamel junction at the base of the defect.

Cemento-enamel junction (CEJ)-Bone crest (BC): The distance of the cemento-enamel junction to the crest of bone.

Cemento-enamel junction (CEJ)- Root apex (RA): The distance of the cemento-enamel junction to root apex.

Lineal Bone Growth (LBG) = CEJ to base of defect at baseline - CEJ to base of defect at 6 months.

%BF: Percent bone fill was calculated by dividing LBG by the depth of the original bone defect

Bleeding on probingInitial and six months after surgery

Method to asses gingival inflammation. Gingival health is defined as \< 10% bleeding sites

Clinical Attachment LevelInitial and six months after surgery

The distance from the cemento-enamel junction to the tip of a periodontal probe during periodontal diagnostic probing, ≥2 mm (millimeters)

Gingival PhenotypeInitial and six months after surgery

Gingival phenotype is determined by gingival thickness and keratinized tissue width.

Probe visible: thin (≤1 mm). Probe not visible: thick (\>1mm).

Probing depthInitial and six months after surgery

The distance from free gingival margin to sulcus depth, ≥4 millimeters (mm)

Plaque control recordInitial and six months after surgery

O'Leary index is used for assessing oral hygiene skills of the patient

Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (1)

Posgrado de Periodoncia, Facultad de Odontología, Universidad Autónoma de Nuevo León

🇲🇽

Monterrey, Nuevo León, Mexico

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