Oral Nitrite Synthesis and Post-exercise Hypotension

Not Applicable

Completed

• Conditions: Blood Pressure; Sports Physical Therapy
• Interventions: Other: Exercise

• Registration Number: NCT03904394
• Lead Sponsor: University of Plymouth

Brief Summary

Exercise is probably the most effective approach to reduce blood pressure. In fact, a single bout of exercise induces a physiological response known as Post-Exercise Hypotension (PEH) where a prolonged decrease in resting blood pressure occurs in the minutes and hours after exercise. However, it is not fully understood how this response triggers. Recent evidence suggests that oral bacteria may play a key role in blood pressure control by enhancing nitrite, and then nitric oxide (NO) bioavailability under resting conditions in humans. However, no previous study has investigated whether this is a key mechanism involve in PEH. Thus, the main aim of this study was to investigate if the oral nitrate/nitrite pathway is a key regulator of PEH and vasodilation in healthy humans.

Detailed Description

Not available

Study Timeline

• Study Start: 2017-05-09
• Primary Completion: 2018-04-20
• Study Completion: 2019-03-11
• Status Verified: 2019-04
• Study First Submitted: 2019-04-03
• Study First Submitted QC: 2019-04-03
• Last Update Submitted: 2019-04-04
• Study First Posted: 2019-04-05
• Last Update Posted: 2019-04-08

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 23

Inclusion Criteria

Blood pressure (SBP < 130 mmHg; DBP < 90 mmHg) BMI < 30 kg/m2 Age 18 to 50 years old

Exclusion Criteria

Smoker Hypertension Dyslipidaemia Diabetes Gingivitis or periodontitis Using antibacterial mouthwash or tongue scrapes Taking antibiotics 1 month before the start of the study Females without have irregular menstrual periods

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
Antibacterial Mouthwash	Exercise	-
Placebo Mouthwash	Exercise	-

Primary Outcome Measures

Name	Time	Method
Change in blood pressure after exercise	Baseline and 1 hour and 2 hours after exercise	Blood pressure was measured before and at 1 hour and 2hours after exercise using an electronic sphygmomanometer (ProBP 3400, Welch Allyn). The second and third readings will be averaged to determine mean blood pressure.

Secondary Outcome Measures

Name	Time	Method
Change in reactive hyperaemia	Baseline and 1 hour and 2 hours after exercise	Tissue oxygenation index (TOI) were recorded on the left forearm (extensor digitorum) using a NIRS system (NIRO-200NX, Hamamatsu) before exercise and 2 hours after exercise. After baseline measurements (2 minutes), an automatic pneumatic cuff (Hokanson E-20 AG101) was inflated \~5cm above the elbow for 5 minutes to an occlusion pressure of 200 mmHg. Then, inflation of the cuff was rapidly released (\< 1 second) and the NIRS measurements were continuously monitored for 5 minutes.

Trial Locations

Locations (1)

Laboratory of Nutrition, Exercise & Health; 🇬🇧 Plymouth, Devon, United Kingdom