Genomic Approaches for Predicting Severity of Organ Dysfunction and Outcomes in Sepsis: a Prospective Cohort Study in Adult Critically Ill Patients With Sepsis
试验速览
- 阶段
- 不适用
- 入组人数
- 120
- 试验地点
- 1
- 主要终点
- blood single cell transcriptome in infection and sepsis
研究概览
简要总结
This is a prospective cohort study using gene expression to study patients with infection and sepsis from pneumonia.
详细描述
This is a prospective cohort study using single cell transcriptomic profiling and plasma DNA tissue mapping on patients with pneumonia with or without sepsis. The major application of the investigator's study would be the discovery of gene expressions in different leucocytes and plasma DNA associated with each type of organ dysfunction in sepsis. These include cardiovascular, respiratory, hepatic, renal, neurological and haematological dysfunction. This would help prediction, diagnosis and development of therapies to treat sepsis. Leucocyte single cell transcriptome and plasma DNA tissue mapping may addresses the limitations of current evidence in 3 ways: (1) differentiate patients with uncomplicated pneumonia versus pneumonia with associated sepsis, (2) correlation with types and severity of organ dysfunction and (3) identifying molecular phenotypes of sepsis.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •All of the following:
- •newly admitted adult patients (≥ 18 years old)
- •suspected community acquired pneumonia (CAP)
- •compatible history of either sputum or cough or fever or rigors within 1 week
- •chest X-ray infiltrates
排除标准
- •Any of the following:
- •chest symptoms not solely accounted by pneumonia (cardiac failure, non cardiogenic pulmonary oedema, suspected pulmonary embolism, suspected secondary acute respiratory distress syndrome)
- •immunosuppression
- •current malignancy
- •blood samples for gene expression could not be taken within 24 hours of admission
- •prisoner/cogni tive impairment
- •blood transfusion within 1 month
- •hospitalization within 1 month
结局指标
主要结局
blood single cell transcriptome in infection and sepsis
时间窗: within 24 hours of hospital admission
comparison of single cell transcriptome between patients with uncomplicated pneumonia and pneumonia with sepsis
次要结局
- blood single cell transcriptome as marker of organ dysfunction(at time points 0, 24 and 72 hours)
- plasma DNA(at time points 0, 24 and 72 hours)
- blood single cell transcriptome as predictor of clinical outcome(at time points 0, 24 and 72 hours)
研究者
Lowell Ling
Clinical Lecturer
Chinese University of Hong Kong