To compare the effect of single versus double dose of recombinant human chorionic gonadotropin on final oocyte maturation in long agonist in vitro fertilisation cycles- A Prospective Randomized Controlled Study

Oocyte maturation is a critical process to the success of in vitro fertilization (IVF) treatment, during which the oocyte gains competence for fertilization. One of the most important predictive factors for the success of IVF is the maturity of oocytes along with number and quality of oocytes available for fertilization. In Long Agonist protocol, there is no endogenous hCG due to suppression of Hypothalamic-Pituitary-Ovarian (HPO) axis. Since, final oocyte maturation and quality of oocytes depend on exogenous hCG given, therefore, we want to find the best dose of r-hCG for obtaining the highest number of mature oocytes after stimulation. Women fulfilling the inclusion criteria will be included in the study and they will undergo treatment with standard long gonadotropin‑releasing hormone (GnRH) agonist protocol. Patients will be randomly allocated into either the 250 or 500 µg r‑hCG group according to a computer‑generated randomization. Transvaginal ultrasound‑guided oocyte retrieval will be performed 36 hours after administration of r‑hCG injection. Primary outcome includes total number of M-II oocytes retrieved per follicle and secondary outcomes include number of good quality embryos generated, biochemical pregnancy rate, clinical pregnancy rate, incidence of Ovarian hyperstimulation syndrome (OHSS) and subgroup analysis in poor responder group.