Biomarkers of Broccoli Intake: A Pilot Study

Not Applicable

Completed

• Conditions: Healthy Volunteers
• Interventions: Other: Broccoli consumption

• Registration Number: NCT03287115
• Lead Sponsor: USDA Beltsville Human Nutrition Research Center

Brief Summary

The objectives of the study are 1) to characterize plasma and urinary metabolites that originate from glucobrassicin, 1-methoxyglucobrassicin, 4-methoxyglucobrassicin, and 4-hydroxyglucobrassicin, which are the major indole glucosinolates found in broccoli, and 2) to identify novel plasma and urinary metabolites following broccoli consumption that may have roles in reducing the risk of cancer.

Detailed Description

Consumption of Brassica vegetables is inversely associated with incidence of several cancers, including cancer of the lung, stomach, liver, colon, rectum, breast, endometrium, and ovaries. Brassicas are a good source of many nutrients, but the unique characteristic of Brassicas is their rich content of glucosinolates. Breakdown products of indole glucosinolates in Brassica vegetables show promise for reducing the risk of cancer, but have received comparatively less research attention than those of aliphatic glucosinolates. The aim of this study is to investigate plasma and urinary metabolites of indole glucosinolates from broccoli and to identify novel metabolites that may have roles in reducing cancer risk.

Study Timeline

• Study First Submitted: 2017-09-15
• Study First Submitted QC: 2017-09-18
• Study First Posted: 2017-09-19
• Status Verified: 2017-11
• Study Start: 2017-11-06
• Primary Completion: 2017-11-17
• Study Completion: 2017-11-17
• Last Update Submitted: 2017-11-20
• Last Update Posted: 2017-11-21

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 6

Inclusion Criteria

• Non tobacco user
• Cancer Free
• Not currently taking glucosinolate/isothiocyanate containing supplements

Exclusion Criteria

• Type 2 diabetes requiring the use of diabetes pills, insulin, or non-insulin shots
• Use of blood-thinning medications such as Coumadin (warfarin), Dicumarol, or Miradon (anisindione)
• History of bariatric surgery or nutrient malabsorption disease (such as celiac disease) or other metabolic disorders requiring special diet recommendations
• Pregnant, lactating, or intending to become pregnant during the study period
• Crohn's disease or diverticulitis
• Suspected or known strictures, fistulas or physiological/mechanical GI obstruction
• Self-report of alcohol or substance abuse within the past 12 months and/or current acute treatment or rehabilitation program for these problems (long-term participation in Alcoholics Anonymous is not an exclusion)

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
Broccoli	Broccoli consumption	Subjects will consume a dose of broccoli on day 11

Primary Outcome Measures

Name	Time	Method
The change in glucosinolate metabolites will be measured in blood plasma and urine.	On Day 11	To track the changes of indole glucosinolate metabolites, glucosinolate metabolites will be measured in both blood plasma and urine.

Trial Locations

Locations (1)

USDA-ARS, Beltsville Human Nutrition Research Center; 🇺🇸 Beltsville, Maryland, United States