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Factorial Randomized Trial of Rendesivir and Baricitinib Plus Dexamethasone for COVID-19 (the AMMURAVID Trial)

Registration Number
NCT04832880
Lead Sponsor
ASST Fatebenefratelli Sacco
Brief Summary

Background:

In the current worldwide medical emergency, a rapid identification of effective therapeutic strategy is crucial. So far, therapy with dexamethasone, remdesivir and baricitinib have been associated with evidence of impact on the clinical impact on COVID-19, but the effect of baricitinib and remdesivir in combination with dexamethasone.

The AAMMURAVID trial is endorced and supported by the Italian Regulatory agency (AIFA-Agenzia Italiana del Farmaco)

Detailed Description

Not available

Recruitment & Eligibility

Status
UNKNOWN
Sex
All
Target Recruitment
4000
Inclusion Criteria
  • Adults aged > 18 years able to provide a valid informed consent to the study

  • Documented COVID-19 by direct testing (positive PCR), with lung infiltrates at imaging (Chest-X ray or CT) and requirement of oxygen supplementation

  • Less than 10 days form symptoms onset

  • Cytokine storm, using the criteria developed at Temple University (all of the three below criteria):

    • CRP > 46 mg/l

    • Ferritin > 250 ng/ml

    • One variable of each of the three clusters below

      • Cluster 1

        • Albumin < 2.8 g/dl
        • Lymphocytes <10.2 % of WBC
        • Absolute neutrophil count > 11400/mm3
      • Cluster 2

        • ALT > 60 U/L
        • AST > 87 U/L
        • D-dimers > 4930 µg/l fibrinogen-equivalent-units (FEU).
        • LDH >416 U/L
        • High sensitivity troponin > 1.09 ng/ml
      • Cluster 3

        • Anion Gap at arterial blood gas < 6.8 mM
        • Chloride > 106 mM
        • Potassium > 4.9 mM
        • BUN:creatinine ratio > 29
  • PaO2/FiO2 200-400 mmHg, while in oxygen therapy or continuous positive airway pressure (C-PAP)

  • For women of childbearing potential and men: agreement to use contraception in the case of heterosexual intercourses before day 28 with a failure rate < 1% per year (bilateral tubal ligation, male sterilisation, hormonal contraceptives inhibiting ovulation, hormone-release or copper intrauterine devices). For men enrolled in the study, condom use is allowed.

Exclusion criteria:

  • Orotracheal intubation or ECMO support

  • Active solid / hematologic cancer (including invasive non-melanoma skin cancer)

  • Hypersensitivity or contra-indications to one of the investigational agents (including history of deep vein thrombosis / pulmonary thromboembolism within 12 weeks prior to screening)

  • Other active concurrent viral, fungal or bacterial infections (including active tuberculosis/latent TB treated for less than 4 weeks, HIV and HCV/HBV infections)

  • Pregnancy/breastfeeding

  • Incapability to provide a valid informed consent (including age < 18 years old)

  • Heart failure with NYHA >= 2 or any acute cardiac or vascular event requiring therapy in the previous 12 months

  • Chronic renal failure (baseline GFR < 45 ml/min*1.73m2)

  • Liver cirrhosis moderate / severe (Child-Pugh B or C)

  • Chronic respiratory failure requiring O2 therapy or ventilation therapy at home

  • Blood neutrophils <1000/mcL, platelet <50000/mcL, Hb levels <80 g/l

  • ALT/AST > 5 times UNL

  • Use of any biologic agent or small molecule inhibitor and other investigational drugs in the previous 4 weeks or 5 half-lives (whichever is longer). Specific cut-offs for wash-out are required for the following therapies:

    • B-cell targeted therapies: 24 weeks or 5 half-lives (whichever is longer)
    • TNF-inhibitors: 2 weeks or 5 half-lives (whichever is longer)
    • JAK-inhibitors: 1 week or 5 half-lives (whichever is longer)
  • Use of other immunosuppressive agents in the last 3 months (chronic use of topical steroids and systemic steroids with a dose ≤5 mg of prednisone equivalents is allowed)

  • Use of any other investigational therapy for COVID-19 (including IV immunoglobulins, convalescent COVID-19 plasma or monoclonal antibodies)

  • Impossibility to discontinue Strong inhibitors of OAT3 (such as probenecid) at study entry

  • Any other condition judged by the local investigator as a contra-indication to eligibility

  • Subjects who have received live vaccines within 4 weeks before the study or are planned to receive live vaccine in the first months after study enrolment.

Exclusion Criteria

Not provided

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Baricitinib armBaricitinib Oral Tablet [Olumiant]IV dexamethasone 6 mg for 10 days + baricitinib 4 mg die for 10 days. For patients aged \> 75 years or estimated GFR \< 60 ml/min\*1.73m2, baricitinib dose is reduced to 2 mg for 10 days.
Remdesivir + baricitinib armBaricitinib Oral Tablet [Olumiant]IV dexamethasone 6 mg for 10 days + remdesivir IV 200 mg on day 1, followed by 100 mg die until day 10 + baricitinib 4 mg die for 10 days. For patients aged \> 75 years or estimated GFR \< 60 ml/min\*1.73m2, baricitinib dose is reduced to 2 mg for 10 days.
Control arm (dexamethasone arm)DexamethasoneIV dexamethasone 6 mg for 10 days
Remdesivir armRemdesivirIV dexamethasone 6 mg for 10 days + remdesivir IV 200 mg on day 1, followed by 100 mg die until day 10
Remdesivir armDexamethasoneIV dexamethasone 6 mg for 10 days + remdesivir IV 200 mg on day 1, followed by 100 mg die until day 10
Baricitinib armDexamethasoneIV dexamethasone 6 mg for 10 days + baricitinib 4 mg die for 10 days. For patients aged \> 75 years or estimated GFR \< 60 ml/min\*1.73m2, baricitinib dose is reduced to 2 mg for 10 days.
Remdesivir + baricitinib armRemdesivirIV dexamethasone 6 mg for 10 days + remdesivir IV 200 mg on day 1, followed by 100 mg die until day 10 + baricitinib 4 mg die for 10 days. For patients aged \> 75 years or estimated GFR \< 60 ml/min\*1.73m2, baricitinib dose is reduced to 2 mg for 10 days.
Remdesivir + baricitinib armDexamethasoneIV dexamethasone 6 mg for 10 days + remdesivir IV 200 mg on day 1, followed by 100 mg die until day 10 + baricitinib 4 mg die for 10 days. For patients aged \> 75 years or estimated GFR \< 60 ml/min\*1.73m2, baricitinib dose is reduced to 2 mg for 10 days.
Primary Outcome Measures
NameTimeMethod
Prevention of very severe respiratory failure or mortalityDay1-Day 28

Composite outcome: Development of very severe respiratory failure (PaO2/FiO2 \<150 mmHg) or mortality

Secondary Outcome Measures
NameTimeMethod
Changes in PaO2 at arterial gas analysisDay 1-28

Course of PaO2 at arterial gas analysis and PaO2/FiO2

Changes in blood troponin TDay 1-28

Course of blood troponin T levels

Prevention of very severe respiratory failure or mortalityDay 21

Composite outcome: Development of very severe respiratory failure (PaO2/FiO2 \<150 mmHg) or mortality

Incidence of Adeverse EventsDay 28

Proportion of number of AEs and SAEs (according to the Common Terminology Criteria for Adverse Events -CTCAE, Version 5.0)

Reduction of the requirements of orotracheal intubation/ECMODay 1-28

Days with orotracheal intubation/ECMO

Velocity in clinical improvementDay 1-28

Time to clinical improvement (defined as one of the following: a) discharge, b) absent ventilator support with NEWS-2 score ≤3 and MELD ≤13)

Velocity in dischargeDay 28

Proportion of discherged patients

Changes in blood ferritinDay 1-28

Course of blood ferritin levels

Changes in blood D-DimerDay 1-28

Course of blood D-Dimer levels

Evolution of the NEWS-2 scoreDay 1-28

Course in the National Early Warning Score-2 score (0-20, with higher scores worse)

Changes in blood creatinine levelsDay 1-28

Course of blood creatine levels

Changes in blood albuminDay 1-28

Course of blood albumin levels

Changes in blood CKDay 1-28

Course of blood CK levels

Fever disappearanceDay 1-28

Time to persistent defervescence persistent defervescence (last day of T\<37.0°C, without recurrent T\>37.0° for at least 4 days)

Changes in periperal blood lymphocytesDay 1-28

Comparison of the course of lymphocytes counts at full blood counts among the treatment arm, as assessed by repeated measures analysis.

Changes in blood hemoglobin levelsDay 1-28

Course of blood hemoglobin

Changes in blood ALTDay 1-28

Course of blood ALT levels

Changes in periperal blood neutrophils countsDay 1-28

Comparison of the course of neutrophils counts at full blood counts among the treatment arm, as assessed by repeated measures analysis.

Changes in blood LDHDay 1-28

Course of blood LDH levels

Changes in blood ASTDay 1-28

Course of blood AST levels

Changes in blood IL-6Day 1-28

Course of blood IL-6 levels

Changes in blood protrombine time (INR)Day 1-28

Course of blood protrombine time (INR)

Changes in blood HDL-colesterolDay 1-28

Course of blood HDL-colesterol levels

Prevention of mortalityDay 1-28

Survival analysis

Prevention of very severe respiratory failureDay 1-28

Time to development very severe respiratory failure (PaO2/FiO2 \<150 mmHg)

Incidence of bacterial/fungal infectionsDay 28

Rate of bacterial/fungal infections

Evolution of the MELD scoreDay 1-28

Course in the Model for End-Stage Liver Disease score (scores \>=6, higher scores worse)

Changes in periperal blood leukocyte numberDay 1-28

Course of periperal blood leukocyte number

Changes in periperal blood plateletsDay 1-28

Comparison of the course of plateletscounts at full blood counts among the treatment arm, as assessed by repeated measures analysis.

Changes in blood bilirubinDay 1-28

Course of blood bilirubin levles

Changes in blood C-reactive proteinDay 1-28

Course of blood C-reactive protein levels

Changes in PaO2/FiO2Day 1-28

Course of PaO2/FiO2

Development of late complications6 months

Proportion of patients with FVC \< 70% of predicted, FEV1 \< 70% predicted and DLCO \< 80% predicted

Changes in blood triglyceridesDay 1-28

Course of blood triglycerides levels

Changes in blood total colesterolDay 1-28

Course of blood total colesterol levels

Trial Locations

Locations (21)

Ospedale di Ferrara

🇮🇹

Ferrara, Italy

ASST Santi Paolo e Carlo

🇮🇹

Milan, Italy

ASST Fatebenefratelli-Sacco

🇮🇹

Milan, Italy

Ospedale San Salvatore

🇮🇹

Pesaro, Italy

Azienda Ospedaliera Integrata -Verona

🇮🇹

Verona, Italy

Policlinico Tor Vergata

🇮🇹

Roma, Italy

Ospedale S Anna

🇮🇹

Como, Italy

Ospedale di Legnago

🇮🇹

Legnago, Italy

Ospedale di Udine

🇮🇹

Udine, Italy

H Goretti

🇮🇹

Latina, Italy

Ospedali Galliera

🇮🇹

Genova, Italy

Ospedale di Legnano

🇮🇹

Legnano, Italy

IRCCS San Raffaele

🇮🇹

Milan, Italy

Ospedali di Prato e Pistoia

🇮🇹

Prato, Italy

Ospedale SS Annunziata -Chieti

🇮🇹

Chieti, Italy

Ospedali Riuniti delle Marche

🇮🇹

Ancona, Italy

Ospedale Parini

🇮🇹

Aosta, Italy

Ospedale di Firenze and Empoli

🇮🇹

Firenze, Italy

Ospedale Manzoni

🇮🇹

Lecco, Italy

Ospedale di Perugia

🇮🇹

Perugia, Italy

Ospedale Cattinara e Maggiore

🇮🇹

Trieste, Italy

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