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FoxBioNet Pilot Project: SAVE (Synuclein Assay Validation Effort)

Completed
Conditions
Parkinson Disease
Registration Number
NCT03170063
Lead Sponsor
Michael J. Fox Foundation for Parkinson's Research
Brief Summary

The overall objective of this study is to compare the performance of available oligomeric and phosphorylated a-synuclein assay in cerebrospinal fluid and blood.

Detailed Description

Specific aims to accomplish this objective are:

1. Assess the reliability of oligomeric and phosphorylated a-synuclein concentration between two different oligomeric and three phosphorylated asynuclein assays.

2. Assess the reliability of the oligomeric and phosphorylated a-synuclein concentrations between laboratories

3. Assess the correlation of oligomeric and phosphorylated a-synuclein concentrations between cerebrospinal fluid and blood.

1.2. Secondary Objectives

1. To assess the ability of the network of pilot sites to efficiently conduct a study involving biosample collection for PD research. Efficiency will be assessed using measures of the time taken to meet specific milestones within the study.

2. To assess the ability of the network to collect high quality biospecimens adhering to agreed-upon protocols.

3. To gauge the willingness of participants to participate in subsequent Fox BioNet studies

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
54
Inclusion Criteria

Parkinson's Disease Subjects

  • Patients must meet the MDS criteria for Parkinson's disease.
  • Disease duration: any
  • Male or female age 30 years or older at time of PD diagnosis.

Control Subjects

  • Male or female age 30 years or older at Screening.
Exclusion Criteria

Parkinson's Disease Subjects

  • Inability to provide informed consent
  • Current treatment with anticoagulants (e.g., coumadin, heparin) that might preclude safe completion of the lumbar puncture.
  • Condition that precludes the safe performance of routine lumbar puncture, such as prohibitive lumbar spinal disease, bleeding diathesis, or clinically significant coagulopathy or thrombocytopenia.
  • Participation in a blinded clinical trial of any kind or an unblinded trial of an investigational product that is not currently approved for use in humans.

Control Subjects

  • Inability to provide informed consent
  • Current treatment with anticoagulants (e.g. coumadin, heparin) that might preclude safe completion of the lumbar puncture.
  • Condition that precludes the safe performance of routine lumbar puncture, such as prohibitive lumbar spinal disease, bleeding diathesis, or clinically significant coagulopathy or thrombocytopenia.
  • Participation in a blinded clinical trial of any kind or an unblinded trial of an investigational product that is not currently approved for use in humans.
  • The presence of rest tremor, bradykinesia or rigidity.
  • The presence of any other neurological sign that in the opinion of the site investigator raises suspicion for an atypical parkinsonian syndrome (e.g. supranuclear gaze palsy)

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
Oligomeric and PS129 α-syn levels3 Months

CSF, serum, and plasma will be analyzed using oligomeric and pS129 assays. The outcome will be expressed as a concentration of modified (pS129 or oligomeric) synuclein or as a ratio of specific species to total synuclein levels.

Secondary Outcome Measures
NameTimeMethod
Time from central IRB approval to site approval (for those sites requiring administrative review)3 Months

To assess time taken from approval by IRB to approval by internal boards for sites.

Time from site selection to contract full execution3 Months

To assess the ability of the network of pilot sites to efficiently conduct a study involving biosample collection for PD research. Efficiency will be assessed using measures of the time taken to meet specific milestones within the study.

Proportion of samples conforming to collection, processing and shipping protocols.3 Months

To assess the ability of the network to collect high quality biospecimens adhering to agreed-upon protocols.

Proportion of participants agreeing to be contacted for future Fox BioNet protocols3 Months

To gauge the willingness of participants to participate in subsequent Fox BioNet studies

Time from IRB submission to approval by central IRB3 Months

To assess time taken from submission of proposal to IRB to approval by the IRB

Time from site activation to recruitment of 10 participants3 Months

Time taken from site activation to recruitment of 10 participants

Trial Locations

Locations (5)

Rush University Medical Center

🇺🇸

Chicago, Illinois, United States

University of Pennsylvania

🇺🇸

Philadelphia, Pennsylvania, United States

Beth Israel Deaconess Medical Center

🇺🇸

Boston, Massachusetts, United States

Oregon Health and Sciences University

🇺🇸

Portland, Oregon, United States

Baylor College of Medicine

🇺🇸

Houston, Texas, United States

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