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Clinical Trials/NCT07300943
NCT07300943
Recruiting
Phase 1

A Phase 1/2 Study of CLIO-8221 in Patients With Advanced Solid Tumors

Callio Therapeutics13 sites in 2 countries306 target enrollmentStarted: March 19, 2026Last updated:
InterventionsCLIO-8221

Overview

Phase
Phase 1
Status
Recruiting
Sponsor
Callio Therapeutics
Enrollment
306
Locations
13
Primary Endpoint
Type, incidence, severity, and seriousness of adverse events (AEs)

Overview

Brief Summary

The study will be conducted in 2 phases: Phase 1: Dose-escalation and Dose Level Expansion, Phase 1 will determine the maximum tolerated dose (MTD) and/or recommended dose for expansion (RDE). Phase 2: Tumor-Specific Expansions with Dose Optimization, Phase 2 will further evaluate CLIO-8221 in tumor-specific expansion cohorts to optimize dosing and assess preliminary efficacy.

Detailed Description

Phase 1: Dose-escalation and Dose Level Expansion. Dose escalation safety data will be reviewed by a Safety Monitoring Committee (SMC) to guide dosing decisions. Backfill enrollment may be used to further characterize safety, PK/PD, and antitumor activity.

Phase 2: Tumor-Specific Expansions with Dose Optimization. Phase 2 will further evaluate CLIO-8221 in tumor-specific expansion cohorts to optimize dosing and assess preliminary efficacy. Safety, tolerability, PK/PD, and response data will support selection of the recommended Phase 2 dose (RP2D) for further development.

Study Design

Study Type
Interventional
Allocation
Na
Intervention Model
Sequential
Primary Purpose
Treatment
Masking
None

Masking Description

No, this is an open-label trial

Eligibility Criteria

Ages
18 Years to — (Adult, Older Adult)
Sex
All
Accepts Healthy Volunteers
No

Inclusion Criteria

  • Patients with advanced solid tumors
  • Patients must have metastatic or unresectable disease not suitable for further local treatment and should have received prior beneficial therapies unless ineligible, unwilling, or lacking access.
  • LVEF ≥50% by echocardiogram (ECHO) or multigated acquisition (MUGA) scan.
  • An Eastern Cooperative Oncology Group (ECOG) Performance Status score of 0 or 1
  • Measurable disease per RECIST version 1.1 at baseline

Exclusion Criteria

  • Prior anti-tumor treatment with an ATRi.
  • Prior or concurrent malignancy whose natural history or treatment has the potential to interfere with the safety or efficacy assessment of the investigational regimen. Exceptions are malignancies with a negligible risk of metastasis or death (e.g., 5-year OS ≥90%), including, but not limited to, adequately treated carcinoma in situ of the cervix, non-melanoma skin carcinoma, localized prostate cancer, ductal carcinoma in situ, and Stage I uterine cancer.
  • History of uncontrolled seizure disorders or clinically significant neurodegenerative disorders, including progressive peripheral neuropathy. Stable Grade ≤ 2 peripheral neuropathy is allowed.
  • Clinically significant autoimmune disease, either currently present or present within the previous 2 years, including a current requirement for systemic immunosuppressive therapy equivalent to \>10 mg/prednisone daily (local immunosuppressive therapy such as inhaled or topical corticosteroids is allowed).
  • Any uncontrolled Grade ≥ 3 (per NCI CTCAE version 6.0) viral, bacterial, or fungal infection within 2 weeks prior to Cycle 1 Day
  • Routine antimicrobial prophylaxis is permitted.
  • History of hepatic cirrhosis, autoimmune hepatitis, or drug-associated hepatitis within the past 12 months.
  • Uncontrolled diabetes mellitus, defined as Hgb A1c ≥8% or Hgb A1c between 7% and \<8% with associated diabetes symptoms (polyuria or polydipsia) that are not otherwise explained.
  • Any other medical, social, or psychosocial factors that, in the opinion of the investigator, could impact safety or compliance with study procedures.
  • Additional protocol defined inclusion/exclusion criteria may apply

Arms & Interventions

Dose escalation and dose level expansion

Experimental

Phase 1: CLIO-8221 monotherapy in escalating doses. Phase 2: Phase 2 will be initiated in tumor-specific expansion cohorts at selected doses.

Intervention: CLIO-8221 (Drug)

Outcomes

Primary Outcomes

Type, incidence, severity, and seriousness of adverse events (AEs)

Time Frame: Through end of treatment, up to approximately 2 years.

Type, incidence, severity, and seriousness of AEs occurred

Type, incidence, severity, and seriousness of adverse events (AEs)

Time Frame: Through end of treatment, up to approximately 2 years.

Type, incidence, severity, and seriousness of AEs occurred

Type, incidence, and severity of laboratory abnormalities

Time Frame: Through end of treatment, up to approximately 2 years.

Type, incidence, and severity of laboratory abnormalities occurred

Incidence of dose limiting toxicities dose (RP2D) of CLIO-8221

Time Frame: From first dose through study day 21.

Incidence of dose limiting toxicities occurred

Secondary Outcomes

  • Progression-free survival(Up to approximately 2 years.)
  • Objective Response Rate(Through disease progression, up to approximately 2 years.)
  • Disease control rate(Through disease progression, up to approximately 2 years.)
  • Duration of objective response(From the date of enrollment until a confirmed partial or complete response is achieved, assessed up to 2 years.)
  • Pharmacokinetic Parameter Area Under the Curve (AUC) for CLIO-8221(Varying timepoints through end of treatment, up to approximately 2 years.)
  • Pharmacokinetic Parameter Maximum Concentration (Cmax) for CLIO-8221(Varying timepoints through end of treatment, up to approximately 2 years.)
  • Pharmacokinetic Parameter Time to Maximum Concentration (Tmax) for CLIO-8221(Varying timepoints through end of treatment, up to approximately 2 years.)
  • Pharmacokinetic Parameter Total Clearance (CL) for CLIO-8221(Varying timepoints through end of treatment, up to approximately 2 years.)
  • Pharmacokinetic Parameter Volume of distribution at steady state (Vd) for CLIO-8221(Varying timepoints through end of treatment, up to approximately 2 years.)
  • Pharmacokinetic Parameter Apparent Terminal Half-life (t1/2) for CLIO-8221(Varying timepoints through end of treatment, up to approximately 2 years.)

Investigators

Sponsor
Callio Therapeutics
Sponsor Class
Industry
Responsible Party
Sponsor

Study Sites (13)

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