Study of Role of Mononuclear Cells of Maternal Peripheric Blood in Human Myometrial Primar Cell Lines Differentiation Toward a Contratyl Phenotype: Influence of Preconceptional Obesity
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 38
- 试验地点
- 2
- 主要终点
- Measuring the expression level of markers (Gama actine, Desmine, Connexine-43, and Ocytocine receptor) by Westernblotting
研究概览
简要总结
The purpose of this study is to improve the understanding of cellular mecanism involved in delivery disorders in obese women, first by characterizing the hyperplasia phenomenon of myometrail cells induced by inflammatory stimulation (Il-1B), then by studying the role of blood mononuclear cells, of leptine and of obese women sera to interfere with the transition of myometrial cells toward hyperplasia phenotype.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- Female
- 接受健康志愿者
- 是
入选标准
- •Informed consent signed
- •Patient affiliated with a social security scheme
- •In front of for obstetrical reasons to give birth by caesarean section (scheduled caesarean section)
- •Or a woman who is not obese in labour and needs to undergo a cesarean section
- •No obvious infectious context
- •Age > 18 years old
排除标准
- •Obvious inflammatory or autoimmune diseases
- •Suspected or confirmed infectious context, including virale infection VIH, VHC, VHB
- •Coagulation disorders
研究组 & 干预措施
pre-conceptional obesity with a scheduled cesarean section
干预措施: Myometrium biopsy and blood samples (Other)
non-obese women with a scheduled cesarean section
干预措施: Myometrium biopsy and blood samples (Other)
women entering labour
women entering labour for vaginal delivery and for vaginal delivery
干预措施: Myometrium biopsy and blood samples (Other)
结局指标
主要结局
Measuring the expression level of markers (Gama actine, Desmine, Connexine-43, and Ocytocine receptor) by Westernblotting
时间窗: up to 2 months
Demonstrate in vitro the capacity of a Il-1B stimulus to induce the differentiation of the myometrial cells toward a hyperplasia phenotype, and that the answer depends on whether this is cells from normal weight women or obese women before conception.
次要结局
未报告次要终点