Changes in Serum Fibroblast Growth Factor 21 (FGF21) Levels Correlated With Exercise

Not Applicable

Completed

Conditions: Sedentary Lifestyle

Interventions: Other: Supervised Exercising

Registration Number: NCT01512368

Lead Sponsor: Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran

Brief Summary: We evaluated the effect of two weeks of intensive supervised physical activity in serum fibroblast growth factor 21 (FGF21) levels.

Detailed Description: Comparative and longitudinal study. Anthropometric and biochemical evaluation were done before, 1hr and 4hr after a bout of exercise and repeated after two weeks of daily supervised exercise. We studied sedentary young healthy women. A treadmill exercise test (following the Bruce's protocol) was done five times per week (from Monday to Friday) for two weeks. The Department of Endocrinology and Metabolism at the INCMNSZ performed all biochemical laboratory measurements using standardized procedures.

Recruitment & Eligibility

Status: COMPLETED

Sex: Female

Target Recruitment: 82

Inclusion Criteria

Women
Aged 18 to 35 years old,
Body mass index (BMI) <30 kg/m2
Without contraindication for exercising

Exclusion Criteria

Metabolic syndrome
Diabetes
Dyslipidemia
Hypertension
Asthma
Thyroid disease
Treated with fibrates, beta agonists or blockers
History or current arrhythmias, murmurs, cardiomegaly, or treatment for cardiovascular diseases -
Contraindications for exercise testing were also considered as exclusion criteria (see references)

Study & Design

Study Type: INTERVENTIONAL

Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
Supervised exercising	Supervised Exercising	A treadmill exercise test (following the Bruce's protocol) was done five times per week (from Monday to Friday) for two weeks to participants.

Primary Outcome Measures

Name	Time	Method
Change From Baseline Fibroblast Growth Factor 21 (FGF21) Acutely and at Two Weeks	Baseline, 1hr and 4 hr after a bout of exercise, and repeated after two weeks of daily supervised exercise	Serum measurement of FGF21 using human ELISA kit was done at baseline, 1hr and 4 hr after a bout of exercise, and repeated after two weeks of daily supervised exercise.

Secondary Outcome Measures

Name	Time	Method
Change From Baseline Free Fatty Acids (FFAs) Acutely and at Two Weeks	Baseline, 1hr and 4 hr after a bout of exercise, and repeated after two weeks of daily supervised exercise	Serum measurement of free fatty acids (FFAs) was done at baseline, 1hr and 4 hr after a bout of exercise, and repeated after two weeks of daily supervised exercise.
Change From Baseline Epinephrine Acutely and at Two Weeks	Baseline, 1hr and 4 hr after a bout of exercise, and repeated after two weeks of daily supervised exercise	Serum measurement of epinephrine was done at baseline, 1hr and 4 hr after a bout of exercise, and repeated after two weeks of daily supervised exercise.
Change From Baseline Leptin Acutely and at Two Weeks	Baseline, 1hr and 4 hr after a bout of exercise, and repeated after two weeks of daily supervised exercise	Serum measurement of leptin was done at baseline, 1hr and 4 hr after a bout of exercise, and repeated after two weeks of daily supervised exercise
Change From Baseline Total Adiponectin Acutely and at Two Weeks	Baseline, 1hr and 4 hr after a bout of exercise, and repeated after two weeks of daily supervised exercise	Serum measurement of total adiponectin was done at baseline, 1hr and 4 hr after a bout of exercise, and repeated after two weeks of daily supervised exercise
Change From Baseline Fasting Glucose Acutely and at Two Weeks	Baseline, 1hr and 4 hr after a bout of exercise, and repeated after two weeks of daily supervised exercise	Serum measurement of glucose was done at baseline, 1hr and 4 hr after a bout of exercise, and repeated after two weeks of daily supervised exercise