Discovery of Biomarkers for Intrinsic Radiation Sensitivity in Cancer Patients
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Nasopharyngeal Cancer
- Sponsor
- National Cancer Centre, Singapore
- Enrollment
- 5000
- Locations
- 1
- Primary Endpoint
- To identify proteins that are associated with radiation sensitivity in tissue samples
- Status
- Recruiting
- Last Updated
- 10 months ago
Overview
Brief Summary
Patients with cancers that are sensitive to radiotherapy treatment and/or patients who have experienced severe acute/ late side effects to radiotherapy will be recruited to the study. Blood and/or matched tumour-normal tissue pairs will be collected. Blood and/or tissue samples will be processed and studied for genetic and biochemical markers that have potential to be used for predicting sensitivity to radiation.
Investigators
Eligibility Criteria
Inclusion Criteria
- •Patients with severe side effects from radiotherapy
- •Patients with a type of cancer that is associated with sensitivity to radiotherapy
Exclusion Criteria
- •Age of patient must be between 21 (inclusive) and 99 (exclusive)
Outcomes
Primary Outcomes
To identify proteins that are associated with radiation sensitivity in tissue samples
Time Frame: Tissue is from archived sample
Immunohistochemistry will be used to detect protein expression
Change in immune profile of blood and tissue after radiation
Time Frame: Blood is taken at informed consent; tissue is from archived sample
Mass cytometry using CyTOF technology will be used to detect the immune cell population
To identify genes or molecular pathways that are associated with radiation sensitivity in blood and tissue samples
Time Frame: Blood is taken at informed consent; tissue is from archived sample
Whole exome sequencing, single nucleotide polymorphism (SNP) assays
To identify RNA expression pathways that are associated with radiation sensitivity in blood and tissue samples
Time Frame: Blood is taken at informed consent; tissue is from archived sample
RNA sequencing