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Autologous Platelet-Rich Plasma Intra-ovarian Infusion in Poor Responders

Phase 2
Recruiting
Conditions
Ovary; Anomaly
Infertility, Female
Interventions
Biological: Placebo
Registration Number
NCT03937661
Lead Sponsor
Genesis Athens Clinic
Brief Summary

Autologous PRP intra ovarian infusion may improve ovarian response, patients' hormonal profile as well as fresh embryo transfer (ET)-ICSI cycles' outcome in patients presenting with Poor Ovarian Response (POR).

Detailed Description

This triple-blind Randomized Controlled Trial (RCT) aims to investigate the effectiveness of autologous PRP intra ovarian infusion on improving ovarian tissue functionality and fresh ET-ICSI cycles' performance in regard to patients presenting with POR. PRP is blood plasma prepared from fresh whole blood that has been enriched with platelets. It is collected from peripheral veins and contains several growth factors such as vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), platelet derived growth factor (PDGF), transforming growth factor (TGF) and other cytokines all of which stimulate tissue proliferation and growth. PRP has been employed in several medical conditions in Orthopedics, Dermatology, and Ophthalmology for wound healing. It's efficacy in ovarian rejuvenation and reactivation and endometrial regeneration has not been fully elucidated. This study aims to investigate the effect of autologous PRP intra ovarian infusion on improving ovarian tissue functionality in POR patients.

Recruitment & Eligibility

Status
RECRUITING
Sex
Female
Target Recruitment
100
Inclusion Criteria
  • Poor Ovarian Response according to Bologna Criteria (fulfilling 2 out of 3 of the following)
  • Age ≥ 40 years
  • AMH < 1.1 ng/ml OR AFC < 7
  • ≤ 3 oocytes with a conventional stimulation protocol)
  • Discontinuation of any complementary/adjuvant treatment including hormone replacement, acupuncture, and botanotherapy, for at least three months prior to recruitment.
  • Willing to comply with study requirements
Exclusion Criteria
  • Any pathological disorder related to reproductive system anatomy
  • Cycle irregularities
  • Amenorrhea
  • Endometriosis
  • Adenomyosis
  • Fibroids and adhesions
  • Infections in reproductive system
  • Current or previous diagnosis of cancer in reproductive system
  • History of familiar cancer in reproductive system
  • Severe male factor infertility
  • Prior referral for PGT
  • Ovarian inaccessibility -Endocrinological disorders (Hypothalamus-
  • Pituitary disorders, thyroid dysfunction, diabetes mellitus, metabolic syndrome)
  • BMI>30 kg/m2 or BMI<18.5 kg/m2
  • Systematic autoimmune disorders

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Group of participants receiving PRP treatmentPRPWomen presenting with POR, treated with autologous PRP intra ovarian infusion and undergoing a subsequent fresh ET-ICSI cycle on the third month of the follow-up period.
Control Group: participants receiving Platelet Free PlasmaPlaceboWomen presenting with POR, treated with autologous PFP intra ovarian infusion and undergoing a subsequent fresh ET-ICSI cycle on the third month of the follow-up period
Primary Outcome Measures
NameTimeMethod
Number of oocytes retrieved34-36 hours following hCG administration

Number of oocytes retrieved following COS in the first fresh ICSI-ET cycle following intervation performed on the third month of the follow-up period

AMH levelsFollow-up period of three months entailing monthly evaluation

Serum AMH levels evaluated monthly for three consecutive months

Secondary Outcome Measures
NameTimeMethod
Antral Follicle CountFollow-up period of three months entailing monthly evaluation

AFC evaluated monthly, on day 2 of the menstrual cycle, for three consecutive months

Clinical pregnancy rate6-7 weeks following last menstruation

Clinical pregnancy rate, following the first fresh ET-ICSI cycle performed on the third month of the follow-up period

Trial Locations

Locations (1)

Genesis AC

🇬🇷

Athens, Greece

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