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The Intervention of Obesity in Children With Prader-Willi Syndrome Using Prebiotics and Probiotics

Not Applicable
Recruiting
Conditions
Prader-Willi Syndrome
Interventions
Dietary Supplement: probiotics including B. lactis B420, B. lactis HN019, B. animalis Bb-12, L. rhamnosus GG
Dietary Supplement: Prebiotics with galactomannan and oligofructose
Registration Number
NCT05791604
Lead Sponsor
Children's Hospital of Fudan University
Brief Summary

Prader-Willi syndrome (PWS) is a rare genetic disease, with hyperappetite and severe obesity. At present, there is no effective drugs and interventions to help control the appetite of PWS patients. More and more evidence has shown that gut microbiota is closely related to obesity. Probiotics and prebiotics can improve the structure of gut microbiota, thus improve blood lipid levels and other biochemical indicators of obese people. Therefore, this study intends to explore the effectiveness and safety of probiotics and prebiotics in controlling appetite and weight gain of PWS children.

Detailed Description

Prader-Willi syndrome (PWS) is a rare genetic disease, with hyperappetite and severe obesity. Morbid obesity and related complications caused by hyperappetite are the most common causes of poor prognosis and death in PWS. At present, there is no effective drugs and interventions to help control the appetite of PWS patients. At present, more and more evidence has shown that gut microbiota is closely related to obesity. Probiotics and prebiotics can improve the structure of gut microbiota, thus improve blood lipid levels and other biochemical indicators of obese people. Therefore, this study intends to explore the effectiveness and safety of probiotics and prebiotics in controlling appetite and weight gain of PWS children. Participants will be divided into three groups, receiving probiotics, probiotics+probiotics or placebo treatment, and observe the changes in body weight, the structure of gut microbiota and metabolic level.

Recruitment & Eligibility

Status
RECRUITING
Sex
All
Target Recruitment
60
Inclusion Criteria
  • Pre-adolescent children with Prader Willi syndrome which were definitely diagnosed by gene testing.
  • Consistent with the diagnostic criteria for obesity.
  • Not participate in other research projects at present or three months before the research;
  • Agree to participate in the test and obtain the consent of their parents; voluntarily be the subjects and sign the informed consent form.
Exclusion Criteria
  • Losing weight in ways other than the intervention measures of this project, such as taking weight loss drugs or known drugs that cause weight change;
  • Use antibiotics within 1 month before the study and lasted for 3 days or more;
  • Use probiotics within 1 month before the study and lasted for 3 days or more;
  • Complicated with liver and renal insufficiency (alanine aminotransferase and serum creatinine indexes exceed 2 times the upper limit of the normal value set by the hospital);
  • Have gastrointestinal diseases affecting food digestion and absorption (such as severe diarrhea, constipation, severe gastrointestinal inflammation, active gastrointestinal ulcer, acute cholecystitis, etc.); severe diarrhea refers to watery stool 3 or more times a day and lasts for 3 or more days. severe constipation refers to defecation 2 or less times a week with difficulty in defecation;
  • Surgery was performed within 1 year before the study (except for appendicitis and hernia surgery);
  • Have hepatitis B, active tuberculosis, AIDS and other infectious diseases;
  • Those who are suffering from mental illness and are taking psychotropic drugs such as antidepressants.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Probiotics and prebiotics groupPrebiotics with galactomannan and oligofructoseTake probiotics and prebiotics during the study
Probiotics groupprobiotics including B. lactis B420, B. lactis HN019, B. animalis Bb-12, L. rhamnosus GGTake probiotics during the study
Probiotics and prebiotics groupprobiotics including B. lactis B420, B. lactis HN019, B. animalis Bb-12, L. rhamnosus GGTake probiotics and prebiotics during the study
Primary Outcome Measures
NameTimeMethod
Body mass index12 weeks

Body mass index is calculated as weight (kg)/\[Height (m)\^2\]. Weight and height will be combined to report BMI in kg/m\^2.

Secondary Outcome Measures
NameTimeMethod
Excessive flatulence4 weeks and 12 weeks

Care givers describe whether excessive flatulence occurs, and if so, provide frequency.

Body composition4 weeks and 12 weeks

Body composition including body fat percentage will be measured through body composition meter.

The structure of gut microbiota4 weeks and 12 weeks

The 16s rRNA genes of fecal samples were analyzed.

Fast total cholesterol level4 weeks and 12 weeks

The fast total cholesterol level will be measured by IMMULITE 1000 (Siemens,Germany).

Weight4 weeks and 12 weeks

The weight is accurate to 0.1kg.

Waist circumference4 weeks and 12 weeks

The waist circumference is accurate to 0.1cm.

Height4 weeks and 12 weeks

The height is accurate to 0.1cm.

Glycosylated hemoglobin level4 weeks and 12 weeks

The glycosylated hemoglobin is accurate to 0.1%.

Fast low density lipoprotein level4 weeks and 12 weeks

The fast low density lipoprotein level will be measured by IMMULITE 1000 (Siemens,Germany).

Fast high density lipoprotein level4 weeks and 12 weeks

The fast high density lipoprotein level will be measured by IMMULITE 1000 (Siemens,Germany).

Fast triglyceride level4 weeks and 12 weeks

The fast triglyceride level will be measured by IMMULITE 1000 (Siemens,Germany).

Defecation status4 weeks and 12 weeks

Care givers describe the defecation status in the past 1 week, including the frequency, nature, and color.

Abdominal pain4 weeks and 12 weeks

Care givers describe whether abdominal pain occurs, and if so, provide frequency.

Body mass index4 weeks

Body mass index is calculated as weight (kg)/\[Height (m)\^2\]. Weight and height will be combined to report BMI in kg/m\^2.

Fast insulin level4 weeks and 12 weeks

The fast insulin level will be measured by IMMULITE 1000 (Siemens,Germany).

Fast glucose level4 weeks and 12 weeks

The fast glucose level is detected by fingertip blood with blood glucose meter.

Trial Locations

Locations (1)

Children's Hospital of Fudan University

🇨🇳

Shanghai, Shanghai, China

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