A Phase 3, Multicenter, Randomized, Double-Masked, Sham-Controlled Clinical Trial to Evaluate the Safety and Efficacy of Gene Therapy for Leber's Hereditary Optic Neuropathy (LHON) Associated With ND4 Mutation
Overview
- Phase
- Phase 3
- Status
- Active, not recruiting
- Sponsor
- Wuhan Neurophth Biotechnology Limited Company
- Enrollment
- 95
- Locations
- 1
- Primary Endpoint
- Efficacy of NR082 in study eye
Overview
Brief Summary
The objective of this clinical study is to evaluate the safety and efficacy of NR082 in the treatment of LHON caused by mitochondrial ND4 gene mutation. This study will enroll subjects aged ≥ 12 years old and ≤ 75 years old to receive a single bilateral intravitreal (IVT) injection of NR082 to evaluate safety and efficacy. The clinical manifestations of all subjects are to be reduced visual acuity caused by LHON associated with ND4 mutation, with laboratory test showing G11778A mutation and reduced visual acuity lasted for >6 months and <10 years.
Detailed Description
Safety run-in phase:
The safety run-in phase will enroll 6 evaluable subjects aged ≥ 12 years and ≤ 75 years , namely 4.5 x 109 vg, 0.05 mL eye/dose（bilaterally） and monitor the safety for at least 6 weeks. If there is no new safety concern evaluated by the SRC, the randomized, double-blind, sham-injection control study can be initiated.
Second Stage: randomized, double-blind, sham-injection control study:
The randomized, double-blind, sham-injection control study is to verify the efficacy and safety of NR082 in LHON caused by mitochondrial gene ND4 mutation . This part is divided into the NR082 treatment group and the control group (sham-injection group).
Study Design
- Study Type
- Interventional
- Allocation
- Randomized
- Intervention Model
- Parallel
- Primary Purpose
- Treatment
- Masking
- Quadruple (Participant, Care Provider, Investigator, Outcomes Assessor)
Eligibility Criteria
- Ages
- 12 Years to 75 Years (Child, Adult, Older Adult)
- Sex
- All
- Accepts Healthy Volunteers
- No
Inclusion Criteria
- •Age at the time of signing the informed consent form: the age of the subjects must be ≥ 12 years old and ≤ 75 years old
- •The clinical manifestation of all subjects is reduced visual acuity caused by LHON associated with ND4 mutation, while the reduced visual acuity lasted for \> 6 months and \< 10 years
- •The clinical manifestation caused by LHON is vision loss, with a visual acuity of ≥ 0.5 LogMAR and ≤1.68 in BCVA in both eye The genotype test result is that there is G11778A mutation in ND4 gene, and there are no other primary LHON-associated mutations in the mitochondrial DNA (mtDNA) (ND1\[G3460A\] or ND6\[T14484C\]) (confirmed by a CLIA-certified international laboratory) Pupils can be adequately dilated for a comprehensive eye examination and visual acuity test
Exclusion Criteria
- •Any known allergy and/or hypersensitivity to the study drug or its constituents Contraindication to IVT injection in any eye
- •IVT drug delivery to any eye within 30 days prior to the screening visit History of vitrectomy in either eye
- •Narrow anterior chamber angle in any eye contra-indicating pupillary dilation
- •Presence of disorders or diseases of the eye or adnexa, excluding LHON, which may interfere with visual or ocular assessments, including optical coherence tomography during the study
- •Presence of known/documented mutations, other than the LHON-related mutation, which are known to cause pathology of the optic nerve, retina or afferent visual system
- •Presence of systemic or ocular/vision diseases, disorders or pathologies, other than LHON, known to cause or be associated with vision loss, or whose associated treatment(s) or therapy(ies) is/are known to cause or be associated with vision loss
- •Presence of optic neuropathy from any cause other than LHON
- •Presence of illness or disease that, in the opinion of the investigator, include symptoms and/or the associated treatments that can alter visual function, for instance cancers or pathology of the CNS, including multiple sclerosis (diagnosis of multiple sclerosis must be based on the 2010 Revisions to the McDonald Criteria) (Polman et al., 2011), and/or diseases or conditions that affect the safety of subjects participating in the study
- •History of recurrent uveitis (idiopathic or immune-related) or active ocular inflammation
- •Participated in another clinical study and receive IP within 90 days prior to the screening visit
Arms & Interventions
Experimental: NR082 injection
4.5E9 viral genomes (vg) , 0.05 mL eye/dose , bilaterally
Intervention: rAAV2-ND4 (Genetic)
Sham Comparator: sham-injection
Sham intravitreal injection （bilateral）will be performed by applying pressure to the eye at the location of a typical intravitreal injection procedure using the blunt end of a syringe without a needle.
Intervention: Sham (No Treatment) (Other)
Outcomes
Primary Outcomes
Efficacy of NR082 in study eye
Time Frame: 52 weeks
Proportion of ≥ 0.3 LogMAR from baseline in BCVA in the study eye in the NR082 treatment and the sham-injection at Week 52 after treatment
Secondary Outcomes
- Efficacy of NR082 in study eye and non-study eye(52 weeks)
- Mean change from baseline in visual field, contrast sensitivity(52 weeks)
- Safety and tolerability of NR082(52 weeks)