Microbiome in Patients With Pulmonary Tuberculosis, Non-tuberculous Mycobacterial Pulmonary Diseases (NTM-PD), Lung Cancer and Hemoptysis
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Tuberculosis, Pulmonary
- Sponsor
- Gachon University Gil Medical Center
- Enrollment
- 43
- Primary Endpoint
- 16S rRNA sequencing
- Status
- Completed
- Last Updated
- 6 years ago
Overview
Brief Summary
Microbiome in lower respiratory diseases is not sufficiently known yet. The objective of this study is to investigate microbiome in patients who present with hemoptysis, and those with pulmonary tuberculosis, non-tuberculous mycobacterial pulmonary disease (NTM-PD), and lung cancer, analyzing respiratory specimen acquired by bronchoscopic approach.
Detailed Description
Subjects who were going to undergo bronchoscopy for hemoptysis or suspected lower respiratory diseases including endobronchial tuberculosis (Tb), NTM-PD, or endobronchial lung cancer (LC) were enrolled after informed consents. Subjects who were supposed to receive bronchoscopy to rule out endobronchial lesions were also enrolled as control group (ctrl) after informed consents. In those control group, endobronchial tuberculosis, malignancy or other certain respiratory diseases was not clearly suspected. Before acquiring respiratory specimens, bronchoscopic channels were washed to acquire negative control in all cases of groups. In Tb group and LC group, specimens of normal bronchial mucosa near suspicious endobronchial lesion were obtained with protected brush (PB), then specimens of abnormal bronchial mucosa in suspicious endobronchial lesion with PB. After acquiring respiratory specimens of PB, bronchial washing was done in suspicious endobronchial lesion. In NTM-PD group, specimens of bronchial mucosa in bronchus of suspicious NTM-PD with PB. After acquiring respiratory specimens of PB, bronchial washing was done in bronchus of suspicious NTM-PD. In hemoptysis group, bronchial washing was done in bronchus of ongoing bleeding. In control group, specimens of bronchial mucosa in predetermined random bronchus (not bronchus with suspicious endobronchial lesion) with PB. After acquiring respiratory specimens of PB, bronchial washing was done in the same bronchus. Microbiome in lower respiratory species was analyzed using 16S rRNA sequencing.
Investigators
Sangmin Lee, MD
Associate professor
Gachon University Gil Medical Center
Eligibility Criteria
Inclusion Criteria
- •Patients who underwent bronchoscopy for hemoptysis, OR
- •Patients who underwent bronchoscopy for suspicious endobronchial pulmonary tuberculosis observed on chest computed tomography, OR
- •Patients who underwent bronchoscopy for suspicious non-tuberculous mycobacterial pulmonary disease (NTM-PD) observed on chest computed tomography, OR
- •Patients who underwent bronchoscopy for suspicious endobronchial lung cancer observed on chest computed tomography, OR
- •Patients who underwent bronchoscopy to rule out endobronchial lesion which did not seem to be typical for any criteria of pulmonary diseases including tuberculosis, NTM-PD or lung cancer on chest computed tomography
Exclusion Criteria
- •Other malignancy, infection or serious diseases of neural, cardiovascular, renal, hepatobiliary, gastrointestinal, hemotologic or respiratory system
- •Use of any antibiotic within a month
- •Vulnerable volunteer
- •Subject's rejection
Outcomes
Primary Outcomes
16S rRNA sequencing
Time Frame: 0 day (the day of study enrollment)
The V1 to V3 regions of the 16S rRNA in respiratory specimens were analyzed for identification of microbiomes.