Detection of transient bacteremia after daily brushing by 16S rDNA Pyrosequencing: a pilot study for method optimizatio
Not Applicable
Recruiting
- Conditions
- healthy subjects
- Registration Number
- DRKS00012397
- Lead Sponsor
- Zahnklinik Uniklinik Köln
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Recruiting
- Sex
- All
- Target Recruitment
- 13
Inclusion Criteria
Patients who regularly (every 6 months) get a dental checkup in the dental clinic of the University Hospital of Cologne
- Patients who belong to the dental clinic's recallsystem and receive a professional teeth cleaning every six months
- Patients who have no diseases
- Patients who have accepted the declaration of agreement and dont take part in another study
- Patients who are older than 18 years
Exclusion Criteria
- Patients who are generally not indicated for dental treatment
- Patients who are regularly take antibiotics
- Patients, for who a experimental bacteremia could be a risk, especially pregnant women or immunosuppressed patients
Study & Design
- Study Type
- interventional
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method An orally induced bacteremia is measured after brushing (after 30 seconds). For this purpose, the oral microbiom of the saliva is measured by means of a dorsal tongue smear before brushing the teeth. This is then compared with the microbiom of the blood after brushing. A blood sampling is carried out after brushing the teeth.
- Secondary Outcome Measures
Name Time Method The microbiom in the blood, triggered by bacteremia by brushing, correlates with the oral microbiome of a dorsal tongue swab.