Clinical, Laboratory, and Biomedical Characterization of Pediatric Acute-onset Neuropsychiatric Syndrome (PANS)

This multi-site study is intended to collect data and samples from a nationwide cohort of children with Pediatric Acute-onset Neuropsychiatric Syndrome (PANS). PANS is defined by three clinical criteria:

1. Abrupt, dramatic onset of obsessive-compulsive disorder (OCD) or severely restricted

food intake AND 2. Concurrent presence of additional neuropsychiatric symptoms, with similarly severe and acute onset, from at least two of the following seven categories:

• Anxiety (particularly separation anxiety)
• Emotional lability and depression
• Irritability, aggression, and/or severely oppositional behaviors
• Behavioral (developmental) regression
• Deterioration in school performance (due to inattention, concentration difficulties, memory deficits, or others)
• Sensory or motor abnormalities
• Somatic signs and symptoms, including sleep disturbances, enuresis, or urinary frequency

3. Symptoms are not better explained by a known neurologic or medical disorder, such as Sydenham chorea, systemic lupus erythematosus, Tourette disorder, or others.

As a clinical syndrome, PANS likely represents a number of different disorders with unique symptom constellations, disease mechanisms, and etiologies. The purpose of this investigation is to identify these distinctive disorders by carefully documenting, archiving, and analyzing the clinical features of each case of PANS, including not only details of the onset and course of symptoms, but also results of physical examination, laboratory assays, and paraclinical assessments, such as electroencephalography (EEG), polysomnography (PSG), brain imaging (e.g., magnetic resonance imaging [MRI] scans), and others. In addition to defining the unique clinical features associated with a specific disorder, we also hope to identify biomarkers of risk and disease severity, including not only determinant genes, but also environmental triggers, such as those producing post-infectious autoimmunity (e.g., Pediatric Autoimmune Neuropsychiatric Disorders Associated with Streptococcal infection, or PANDAS). To accomplish this, we propose to collect and archive clinical information and biospecimens at different stages of the illness (e.g., initial onset, first exacerbation, following known environmental triggers, during convalescence, etc.) and also to compare these results against data and specimens obtained from healthy controls and children with non-PANDAS OCD, tics, anxiety disorders, or eating disorder. Clinical, laboratory, and biomedical data will be stored in the NIMH database (housed on the Clinical Trials Database [CTDB] of NICHD) and blood, genetic material, and other biospecimens, including microbial cultures, stored in the NIMH Genetics/Biological Repository. There are two types of baseline evaluations within this protocol. The first is limited to participants with acute-onset neuropsychiatric symptoms (PANS subjects) and will be conducted in the NIH Clinical Center by investigators from the NIMH Section on Behavioral Pediatrics (Drs. Swedo, Grant, and Hommer.) These in-depth phenotyping evaluations will include medical and psychiatric history, family history, completion of standardized questionnaires, physical and neurological examinations, neuropsychological testing, MRI scan, throat swab (for microbiologic testing), cheek swab (to obtain genetic samples), and phlebotomy to obtain blood for laboratory assays and genetic testing. Children with undiagnosed acute-onset neuropsychiatric symptoms may undergo additional testing as part of the diagnostic evaluation; these assessments might include (among others), electroencephalography (EEG), echocardiogram/electrocardiogram (ECHO/ECG) and/or polysomnography (PSG). If the child s clinical presentation warrants a lumbar puncture as part of the diagnostic evaluation, (e.g. abnormal findings on neurologic exam, EEG or PSG, or lab abnormalities suggestive of systemic autoimmunity or neuroinflammation), a sample of cerebrospinal fluid will be collected for research, as well as for clinical testing. Sedation will not be used for these lumbar punctures, nor will it be used for any research purposes in this protocol.