Correlation of E-Cadherin and Cyclin D1 expression with histomorphological features in Oral Squamous Cell Carcinoma - A cross sectional study

The study will be conducted in Department of Pathology, Rohilkhand  Medical College

and Hospital, Bareilly after taking approval from Institutional Ethics Committee

and consent from patient and will include all oral biopsy specimens received in the

department during the study period.

The received specimen will be subjected to gross and microscopic examination as per

standard protocol.

Paraffin embedded tissues (4-5 micron) will be cut stained by Haematoxylin and Eosin

stain.

For immunohistochemistry, sections will be deparaffinised and by the usage of EDTA

buffer, Heat Induced Epitome Retrieval (HIER) will be done.

At 1:100 dilution incubation of tissues section will be done with ready to use antibody

against IHC markers.

Detection will be done involving 3,3 – diaminobenzene (DAB) detection kit and the

Hydrogen Peroxide Substrate and a DAB chromogen will be used to view markers

antibody staining.

Observations will be made on IHC stained slides and documented.

Immunohistochemical antibodies E cadherin & cyclin D1 of Path n SITU company will be

used.

Prepared slides will be viewed under optical microscope, for positive or negative

immunostaining.