The Role of Probiotics in Preventing Dysbiosis in Periodontal Disease
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 36
- 试验地点
- 2
- 主要终点
- Periodontal probing depth
研究概览
简要总结
In this randomized controlled trial, it was aimed to evaluate the effect of probiotics (chewable tablets) and kefir consumption in the initial periodontal treatment of periodontitis patients on the change of oral microbiota and treatment. In this clinical trial, 36 systemically healthy volunteers who were diagnosed with periodontitis who were admitted to Gazi University Faculty of Dentistry, Department of Periodontology were included. Besides the inclusion criteria, the nutritional habits of the patients were evaluated by questionnaire. For diagnosis, plaque index, gingival index, bleeding on probing, periodontal probing depth and clinical attachment level were examined. Patients were randomly divided into 3 groups; probiotic, kefir and control group. Following the periodontal diagnosis, clinical index records of the patients and subgingival microbial samples were obtained. The supragingival plaque sample was removed and the subgingival microbial dental plaque sample was gently taken with a sterile Gracey curette. In the same session, periodontal initial treatment was started. According to the groups, they were involved simultaneously with the treatment, kefir group was advised to consume kefir as a liquid once a day in 14 days; probiotics group was advised to consume as a chewable tablet once a day in 14 days; the control group was advised without additional food supplements. Periodontal indexes were repeated in the 1st and 3rd months. Subgingival plaque samples were taken at baseline and at 3 months and DNA sequencing was performed for Tanneralla forsythia (T. forsythia), Porphyromonas gingivalis (P. gingivalis), and Treponema denticola (T. denticola).
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Treatment
- 盲法
- Single (Participant)
入排标准
- 年龄范围
- 18 Years 至 70 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Systemically healthy
- •Individuals over 18
- •Periodontitis (4-6 mm pockets) patients with 3 teeth in each half of the jaws except wisdom tooth
- •Not using probiotic tablets/capsules and consuming no more than 3-4 probiotic food.
排除标准
- •Antibiotic use within 6 months.
- •Breastfeeding or pregnancy.
- •Acute oral lesions or necrotizing ulcerative periodontitis
- •Diabetes, rheumatic fever, lung, kidney disease history and drug use that will affect periodontal tissues
- •Patients who smoke or who did not stop until last year
- •Use of probiotics tablets or capsules
- •Probiotic food consumption not more than 3-4 times a week
研究组 & 干预措施
Probiotics
The probiotics group was advised to consume a chewable tablet once a day in 14 days.
干预措施: Probiotics (Dietary Supplement)
Kefir
The kefir group was advised to consume kefir as a liquid once a day in 14 days.
干预措施: AOÇ Kefir (Dietary Supplement)
Control
the control group was advised without additional food supplements.
干预措施: Food without additional food supplements (Other)
结局指标
主要结局
Periodontal probing depth
时间窗: 3rd month
the distance from the gingival margin to pocket base.
Gingival index
时间窗: 3rd month
It is an index that shows the severity and quantity of gingival inflammation.
Microbiological sample
时间窗: 3rd month
Subgingival plaque samples were taken at baseline and at 3 months and DNA sequencing was performed for Tanneralla forsythia, Porphyromonas gingivalis, and Treponema denticola.
Bleeding on probing
时间窗: 3rd month
Bleeding that is induced by gentle manipulation of the tissue at the depth of the gingival sulcus.
Plaque index
时间窗: 3rd month
It is an index that shows the amount of microbial dental plaque on the teeth.
Periodontal probing depth
时间窗: Baseline
the distance from the gingival margin to pocket base.
Periodontal probing depth
时间窗: 1st month
the distance from the gingival margin to pocket base.
Gingival index
时间窗: Baseline
It is an index that shows the severity and quantity of gingival inflammation.
Gingival index
时间窗: 1st month
It is an index that shows the severity and quantity of gingival inflammation.
Microbiological sample
时间窗: Baseline
Subgingival plaque samples were taken at baseline and at 3 months and DNA sequencing was performed for Tanneralla forsythia, Porphyromonas gingivalis, and Treponema denticola.
Microbiological sample
时间窗: 1st month
Subgingival plaque samples were taken at baseline and at 3 months and DNA sequencing was performed for Tanneralla forsythia, Porphyromonas gingivalis, and Treponema denticola.
Plaque index
时间窗: Baseline
It is an index that shows the amount of microbial dental plaque on the teeth.
Plaque index
时间窗: 1st month
It is an index that shows the amount of microbial dental plaque on the teeth.
Bleeding on probing
时间窗: Baseline
Bleeding that is induced by gentle manipulation of the tissue at the depth of the gingival sulcus.
Bleeding on probing
时间窗: 1st month
Bleeding that is induced by gentle manipulation of the tissue at the depth of the gingival sulcus.
次要结局
- Clinical attachment level(3rd month)
- Clinical attachment level(Baseline)
- Clinical attachment level(1st month)